EXPERIMENT STATION BULLETINS. 



285 



XIV. THE ACTION OP 96.5 PER CENT OP CARBON DIOXIDE UPON THE DEVELOP- 

 MENT OF BACTERIA. 



Table XII represents culture plates made in exactly the same way and allowed to 

 develop under identically the same conditions as in Table X, with this exception, the 

 carbon dioxide in the aerating apparatus was reduced to 96.5 per cent. 



TABLE XII. 



119. 

 120. 

 121. 

 122. 



123.. 

 124.. 

 125.. 

 126.. 

 127.. 

 128.. 

 129. 

 130.. 

 131.. 

 132., 

 133. 

 134. 



No. of micro-organism. 



Plates developed in 

 96.5% COa. 



Growth. 



n. g. 



r. g- 

 n. g. 



r- g- 



n. g. 



r. g- 



No. of 

 colonies. 



2,040 



Plates developed in 

 air as check. 



Growth. 



v. g. 



v. g. 



v. g. 



2,640 v. g. 



v. g. 



I 

 1,650 | v. g. 



No. of 

 colonies. 



3,480 

 2,000 

 1,280 

 2,060 

 1,920 

 1,900 



n. g. 

 n. g. 

 r- g- 



3,000 



V. g. 

 v- g- 

 V. g. 



260 



270 



2,800 



r- g- 



r. g. 

 n. g. 

 n. g. 

 n. g. 



200 

 1,200 



V. g- 

 v. g- 

 v. g. 

 v. g. 

 v. g. 



660 

 1,870 

 3,600 

 2,060 

 4,400 



Again the retardation and inhibition of the growth of micro-organisms appear to 

 be about the same as in Table X. No development occurred in 119, 121, 123, 126, 127, 

 132, 133, 134 or eight out of sixteen and 119, 123, 125, 127, 133 were wholly inhibited 

 or even destroyed for they did not develop after exposure to air for 48 hours. The 

 restraining influence therefore was exerted in every instance, extending to total inhibition 

 or destruction in the cases cited. 



