APPENDIX. 495 



24. Sachs's Food-solution for green plants. 



Distilled water 1000 cub. cent. 



Potassium nitrate i gramme. 



Sodium chloride 0-5 gramme. 



Calcium sulphate 0*5 gramme. 



Magnesium sulphate 0-5 gramme. 



Calcium phosphate 0*5 gramme. 



To this solution add a trace of a weak solution of ferric 

 chloride. The calcium phosphate is only slightly 

 soluble in water. 



25. Schulze's Macerating mixture. 



Dissolve I gramme of potassium chlorate in 50 cub. cent. 

 of nitric acid. Immerse the tissue in this solution, and 

 heat it. The tissue will then readily break up into its 

 constituent cells, their middle lamellae being dissolved. 



Cold maceration often gives better results, but takes 

 longer. 



Take care that the gases given off from the mixture 

 do not injure the microscope. 



26. Schulze's Solution. 



Dissolve some zinc in hydrochloric acid ; permit the 

 solution to evaporate, in contact with metallic zinc, until 

 it has attained a syrupy consistence. Saturate the syrup 

 with potassic iodide, and then add enough iodine to 

 make a dark sherry-coloured solution. The object to be 

 stained must be placed in a little water, and then some 

 of the above solution added. 



27. Silver Nitrate, Solution of* 



Dissolve 05 grammes of silver nitrate in 100 cubic cent, 

 of distilled water. Keep in an opaque stoppered bottle. 



28. Sodic Chloride, Solution of. (Normal saline Solution. 

 Salt solution?) 



Dissolve 7*5 grammes of sodic chloride in i litre of 

 distilled water. 



