APPENDIX. 



497 



a. White of egg metJiod. 



Smear the surface of the slide with a thin but uniform 

 fihn of freshly drawn white of ^gg and deposit the 

 sections in order of cutting. Gently heat the slide 

 until the paraffin begins to melt and then put it aside. 

 When set, put the whole bodily into turpentine and 

 leave it until all the paraffin is dissolved out; upon ex- 

 amination the sections will be found to be firmly at- 

 tached to the glass slide by means of the coagulated 

 albumen. 



Immersion in turpentine for an indefinite period 

 will do no harm. 



b. Kreasote and shellac method. 



Smear the surface of the slide with a heated solution 

 of white shellac in Kreasote. Submit the whole to 

 the temperature of the melting point of the paraffin 

 until the kreasote is evaporated, whereupon the 

 sections will become firmly adherent to the glass by 

 means of the shellac. Next immerse the whole in 

 turpentine, and leave it at rest until the excess of im- 

 bedding material is dissolved out. 



This method is best applicable to preparations 

 which have been previously stained and clarified. The 

 white of ^g<g process is not only the simpler of the two, 

 but the more advantageous, as the sections may be cut 

 and mounted unstained, that process and the sub- 

 sequent clarifying being permissible after fixture to the 

 slide. 



4. Final mounting. 



Allow sufficient of the canada balsam to drop upon the 

 sections to fully cover them. When thoroughly diffused 

 among them, smear the under face of a cover-slip with 

 balsam and place one edge of it upon the slide support- 

 ing its body upon the point of a needle ; by gradually 



M. 32 



