FIELDS S-1. S-2, and S-3 

 Columns 54, 55, and 56 



tract into which administration of the test compound is directly made, if it is not the specifically 

 responding part coded in Field H-1. 



Oral route or postoral route, administration of an unmeasured dose (uncontrolled feeding, for 

 example). Use Symbol 4 for ad libitum feeding, but only when no final measure of the intake 

 is made; when a measure of the intake is made after ad libitum feeding, use Symbol 2. Use 

 Symbol N when a treated environment is consumed (or potentially consumed) by the test organ- 

 ism (e.g., cloth impregnated against moth larvae). Field S-3: Almost without exception admin- 

 istration of an unmeasured dose of a test compound is oral, but if it is postoral. Field H-2 can 

 be used with Symbol 4 as it is used with Symbol 3. 



Intravascular injection, unspecified or specified as intravenous, intra- arterial, intraportal, or 

 intracardial. (Use Symbol 5 for injections into closed vascular systems. Use Symbol 6 for 

 invertebrate, "open" vascular systems [e. g. , insect vessels]. ) Field S-3 : With Symbol 5, 

 Field H-2 can be used to distinguish the intravascular injections by coding In it the vein, 

 artery, portal vessel, heart, or other specific vessel to which injection is made, unless that 

 vessel is also the organ specifically responding to the test compound and coded in Field H-1. 



Intraperitoneal injection. Also, injection into an invertebrate body cavity (e. g. , insect body 

 cavity) or into an invertebrate "open" vascular system. Coelomic injection. 



Intramuscular injection. Field S-3 : Field H-2 can be used to specify the body area or specific 

 muscle into which the test compound was injected, if this is not the anatomical part specifically 

 responding coded in Field H-1. 



Subcutaneous injection; intradermal, intracutaneous injection. Use Symbol 8 for iontophoresis 

 and for injection into lymph sacs of frogs. Field S-1 : Use Symbol 8 for exposure to active 

 cutaneous penetration of parasites (larval nematodes, trematodes, insects, etc. ) Field S-3 : 

 Field H-2 can be used to specify the body area into which the test compound was injected, if 

 this is not the anatomical part specifically responding, coded in Field H-1. 



Tracheal injection. 



Intracellular injection. 



Injection in--or topical application on--or dipping of--an EXPOSED OR ISOLATED ORGAN OR 

 TISSUE (when application is single or repeated, but not when it is continuous for which use 

 Symbol C). I. e. , use Symbol B for applications which do not assure exposures (of the isolated 

 or exposed organ or tissue) to a constant level of the test compound. If administration is made 

 by single, non-continuous injection into a vessel of an exposed (but not isolated) organ, use 

 Symbol 5 rather than Symbol B. Also, a single or repeated "injection" into a flowing, but 

 non-circulating liquid perfusing an EXPOSED OR ISOLATED ORGAN OR TISSUE (but not into a 

 static or circulating [i. e. , a closed-system] bathing liquid for which use Symbol C). (Use 

 Symbol C for administration by which the organ or tissue is exposed to a relatively constant 

 quantity--e. g. , administration to a static bath or a closed-system, circulating perfusate 

 [coded in Field J, if the isolated part is of a test organism, or written in Field J, if the isolated 

 part is of a host]. ) Field S-1: Use Symbol B to code an injection of, or topical application of, 

 an inoculum into an exposed or isolated organ or tissue. Field S-3: A specific, exposed (but 

 not isolated) organ to which single or repeated (but not continuous) application is made can be 

 coded in Field H-2, if that organ is not the organ specifically responding to the test compound 

 and coded in Field H-1. 



Application as (or as a constituent of) a perfusion or bath (circulating or non-circulating, open 

 or closed flowing system) of an ISOLATED OR SURGICALLY EXPOSED ORGAN OR TISSUE, thereby 

 providing a relatively constant concentration and continuous exposure . This includes the 

 Warburg technique for an organ, tissue, homogenate, or any enzyme preparation. Symbol C is 

 also used for topical applications to isolated or surgically exposed organs or tissues, such as 

 a salve, smear, ointment, etc. , which provides a constant coat and exposure . (For single or 

 repeated injections to an isolated or surgically exposed organ or tissue, which do not provide 

 a constant dose level, use Symbol B. For topical application to the intact organism, use 

 Symbol G. For immersion of the intact organism use Symbol E (brief immersion) or Symbol N 



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