FIELDS S-1, S-2, and S-3 

 Columns 54, 55, and 56 



( continuous immersion). When the perfusion or bath is not the pure chemical, treat the bathing 

 or perfusing medium (e. g. , normal saline, glucose solution, etc. ) as a host of the organ or 

 tissue: if the organ or tissue is of a test organism, code this medium in Field J, but if the 

 organ or tissue is of a host organism, the medium can only be written in the abstract for Field J. 

 Field S-1: Symbol C as it is defined here is applicable only for Fields S-2 and S-3 and is not 

 intended for use in Field S-1. Application of an inoculum to an exposed or isolated organ or 

 tissue is coded by Symbol B. Field S-3: A specific, exposed (but not isolated) organ to which 

 continuous (but not single or repeated) application is made can be coded in Field H-2, if that 

 organ is not the organ specifically responding to the test compound and coded in Field H-1. If 

 the bath or perfusing medium is coded in Field J, the concentration of the test compound should 

 be coded in Field M with Symbol # in Column 46 (or in Field N, if the dosage is expressed in 

 "units", with Symbol # in Column 48). 



D Administration to a relatively INTACT PLANT by adding the compound to (or substituting the 



compound, if a liquid, for) water, nutrient medium, or soil, so that it is carried through the 



(Plants root, stem, and other parts. Also, use Symbol D for "injection" into plants (ordinarily a 

 only) continuous administration, from a reservoir, through a fixed injection tube or needle). Field 

 S-3: The specific plant organ through which the test compound enters or is injected can be 

 coded in Field H-2, if that part is not the part specifically responding coded in Field H-1. 



E Brief, direct exposure to the surface or part of the surface of the intact organism; for example, 



dipping (i. e. , essentially a surface exposure by relatively brief immersion) of the whole, intact 

 organism or of a specific surface (an organ or tissue forming part of the body surface) of the 

 intact organism. Also, a wash of brief duration, such as eye or nose drops which are rather 

 quickly washed from the mucosal surfaces. (If an organ or tissue is isolated or an internal 

 organ or tissue has been surgically exposed , use Symbol B, rather than Symbol E, to code its 

 being given only brief surface exposure, e. g. , dipping. Symbol E is opposed also to Symbols 

 N and G in that N is used to code prolonged , continuous immersion and perfusion and G is used 

 to code a more or less prolonged exposure to a material applied to the surface as a smear, oint- 

 ment, salve, etc. , even if that surface coating which provides a continuous exposure is applied 

 by dipping. Symbol E also differs from Symbols K and L in that K and L are ordinarily for coding 

 mass applications to a group of organisms [i. e. , to a population]; in any case, K and L are not 

 used to code specific controlled treatment of a single individual organism. ) The dipping medium 

 or wash (solvent or carrier)is never considered to be a host and is not coded in Field J. Field 

 S-1: Use Symbol E to code the introduction of the test organism to the surface of the intact host 

 by relatively brief exposure of the host, such as dipping or washing with the inoculum. If the 

 test organism actually actively penetrates the skin rather than attaches locally, use Symbol 8 

 rather than Symbol E or G. Field S-3; If brief exposure (e. g. , dipping) is restricted to only a 

 specific part (organ or tissue) of the intact organism. Field H-2 can be used to code that 

 specific part, if it is not the part specifically responding coded in Field H-1. 



G Prolonged, continuous exposure by direct application to the surface- -or to some specific part of 



the surface--of the intact individual organism. Such direct surface applications are referred to 

 as topical, local, percutaneous, etc. Examples: inunction, ointment applications, application 

 by lanolin paste, screw worm smears, etc. For such application to excised organs (e. g. , an 

 orange, considering the fruit as an organ), use Symbol B. Symbol G is not used to code 

 immersion of the intact organism for continuous exposure (e. g. , for administration as part of 

 the liquid environment of the organism) for which there is the special Symbol N. Field S-1: 

 Use Symbol G to code the introduction of the test organism to the surface of the intact host by 

 relatively protracted exposure of the host, --for example, by forcing the host to stand partly 

 submerged in the inoculum. If the test organism actually actively penetrates the skin rather 

 than attaches locally, use Symbol 8 rather than Symbol E or G. Field S-3: In the case of 

 Symbol G, the application is usually restricted to the area coded in Field H-1 rather than to the 

 entire surface of the organism. However, if application is to the entire body surface (Symbol A 

 of Field H) or to a restricted area other than the area coded in Field H-1, that area (organ or 

 body region) should be coded in Field H-2. 



I Administration through respiratory organs. Field S-3: If the test compound is administered to a 



specific part of the respiratory tract, that part should be coded in Field H-2 if it is not the 

 organ specifically responding in Field H-1. 



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