FIELDS S-1, S-2. and S-3 

 Columns 54, 55, and 56 



J Fumigation (i. e. , administration as a gas, aerosol, or mist) of the HABITAT with the organism 



present . Under the conditions for which Symbol J should be used, the administration is not 

 implicitly by inhalation (Symbol I), nor does the administration involve determined direct 

 application to the surface of the organism (Symbols K and L), nor is the administration to an 

 environment prior to the organism's contact with the surface of the habitat (Symbol M). The 

 administration to a habitat in which the administered material is absorbed or mixed, i. e. , a 

 habitat through which it is dispersed and therefore diluted, is coded by Symbol N, except that 

 if this is accomplished by the process of fumigation and the organism is present at the time of 

 fumigation. Symbol J should be used. Field S-1: It is possible that administration of the test 

 organism (microorganisms) might be made by a process of dispersing a suspension of the organ- 

 ism in a mist, etc. (i. e. , by a dispersion analogous to "fumigation" with a test compound or 

 secondary compound). For such an administration of the test organism. Symbol J should be used. 



K Direct application to both the surface of an organism (implying the total surface, not any specific 



part of the surface) and its environment when the dose per individual is not controlled and the 

 organism is not removed from the treated environment. For example, spraying of PLANTS and 

 the soil and other environmental components of the plants, or spraying of INSECTS and the plants 

 on which the insects are as well as other environmental components of the insects, etc. , when 

 the organisms are not removed from the treated environment. Use Symbol L for this application 

 method, when the treated organism is removed from the environment. See the definitions for 

 Symbols G, J, and N to further distinguish the uses of Symbol K. 



L Application as defined for Symbol K, but the treated organism is removed from the environment 



after the treatment . Examples: Peet-Grady fly spray technique, certain settling tower methods, 

 etc. See the definitions for Symbols E and J to further distinguish the uses of Symbol L. 



M Application to the habitat prior to the organism's contact with the SURFACE of that environment. 



(The application may be either to the surface only [a glass plate, e. g. ]--or to an absorbent 

 environmental material [paper, e. g. , ] which nevertheless retains on its surface some of the 

 applied compound. ) Examples (Fields S-2 and S-3): the panel test for house flies, an appli- 

 cation to foliage or other surface for residue tests, impregnated paper barriers for rodent 

 repellency (also other similar repellency tests), the apple-plug technique, a pre-emergency 

 herbicide test, etc. (Use Symbol N or J when the application is to an environment throughout 

 which the applied material is dispersed and IN which ( not on which) the organism is, or will be, 

 living and which the organism may consume as food. Use Symbols K or L when the application 

 is to the surface [or mixed with] the environment with the organism present . ) (Note: For 

 Field S-1, it is so improbable that an inoculum would be administered to a host by such a 

 circuitous route as that described by Symbol M, the symbol is considered as inapplicable to 

 Field S-1. ) 



N Application to the habitat throughout which the applied material is dispersed and diluted (i. e. , 



impregnation of, mixing with, diffusion in, saturation of the habitat), in which the intact 

 organism is, or will be, living (mosquito larvae in water or nematodes or plant seeds in soil) 

 and which it may consume (moth larvae in cloth). Examples: wood impregnation as protection 

 against or treatment for infesting organisms (termites, fungi, marine borers, etc. ), treatment 

 of flour or grain for action against weevils or other infesting organisms (except by fumigation 

 for which Symbol J is specially provided), addition to water in which aquatic organisms are 

 living, addition to media hi which are microorganisms, etc. (If the organism contacts only 

 the surface of the habitat subsequent to application , use Symbol M. Symbol M is always used 

 for repellency studies involving such applications. If the application as described for Symbol N 

 is by fumigation and the organism is present at the time of application, use Symbol J which is 

 specially provided for this. Symbol N differs from Symbol K in that Symbol K refers to appli- 

 cations which are essentially to the test organism and Incidentally to whatever structures and 

 materials of the habitat on which the applied material falls (there is implication that the 

 environment has the applied material evenly distributed in it by diffusion, saturation, or 

 impregnation), whereas Symbol N refers to applications essentially to the habitat through which 

 the applied material is diffused to reach the organism. If the applied material is added to a 

 liquid and this mixture Is applied only briefly as a dip or wash, use Symbol E. ) 



Administration to a parent organism containing- -or to which is attached- -its developing young, 



when the test is for effects on the embryo and/or offspring. (If administration is directly to 



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