430 



RESEARCHES ON FUNGI 



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placed gemmae on living Coffee leaves and observed that a typical 

 leaf -spot was formed beneath and around each gemma, thus proving 



beyond all doubt that the 

 gemmae serve as sources of 

 infection. Presumably, Kohl 

 saw new gemmifers arise on 

 his experimental leaf-spots, 

 although he does not mention 

 them. 



In the autumn of 1925, 

 T. C. Vanterpool and the writer, 

 working in conjunction at 

 Winnipeg, succeeded in in- 

 fecting a number of living 

 leaves with Omphalia jiavida. 

 One set of the experiments 

 will now be described. 



Two leaves of each of the 

 following plants were gathered 

 from the green-house : Bryo- 

 phyllum calycinum, Nerium 

 Oleander, and a species of Ficus. 

 The leaves were then washed 

 for about two minutes in 

 • 1 per cent, mercuric chloride, 

 rinsed in distilled water, and 

 set on very wet sand in a large 

 crystallising dish. One leaf of 

 each kind was then wounded 

 by scratching it very slightly 

 with a sterilised needle, and 

 the other leaf of each kind 

 was left intact. Then a very 

 few gemmae were removed from 

 a bread culture with the help of a sterilised needle and placed in 

 particular spots on the upper surface of all the six leaves. In the 

 case of the three wounded leaves the gemmae were placed on the 



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