OMPHALIA FLAVIDA 



433 



Melastomaceae {unidentified spp.) . 

 Moraceae (Ficus) 



Plumbaginaceae {Plumbago) . 

 Rosaceae {Eriobotrya japonica — 



Loquat) 



{{Coffea) 



1 {Cinchona) . 



Rubiaceae 



Rutaceae 



{Orange) 



Maublanc and Rangel. 

 Buller and Vaiiterpool. 

 Buller and Vanterpool. 



Maublanc and Rangel. 

 Cooke and others. 

 Kohl.i 

 Fawcett. 



Araceae 

 Commelinaceae 



Musaceae 



MONOCOTYLEDONES 



( Yautia) . . . Fawcett. 



{Commelina spp.) . . Fawcett. 



{Zebrina pendula) . . Miiller.^ 



{Banana) 



. Fawcett. 



Various Ferns 



Ptertdophyta 



{unidentified) . . Fawcett. 



Sterility of the Mycelium Induced by Prolonged Cultivation on 

 Artificial Media. — S. F. Ashby isolated his pure culture of Omphalia 

 flavida in Trinidad in 1925. Subcultures of this culture were used 

 by Vanterpool and myself in the winter of 1925-1926, during which 

 time the mycelium fruited well both on artificial media and on living 

 leaves. Two years later, in the winter of 1927-1928, I procured 

 another subculture of Ashby 's pure culture from the Centralbureau 

 voor Schimmel-cultures at Baarn, but found that the mycelium on 

 bread and agar media, even after many weeks, produced neither 

 gemmifers nor sporophores. This sterility appeared to be due to 

 prolonged cultivation of the mycelium on artificial media. 



The mycelium which, instead of producing gemmifers, developed 

 an aerial fluffy hyphal mat was brought back into the fruiting condi- 

 tion by passing it through living leaves. Some Bryophyllum leaves, 

 kept on wet sand in Petri dishes, were wounded by scratching with 



^ F. G. Kohl, loc. cit., pp. 66-68. Kohl, in Central America, examined 36 different 

 kinds of plants and found ten of them affected by the leaf-spot disease. One was 

 a Quinahautn. The others he was unable to identify. 



2 A. S. Miiller, at Mayagiiez, Porto Rico, in litt., 1928. 



VOL. VI. 2 F 



