xii List of Illustrations 



PAGE 



Fig. 27. Diagram of special pierced slide for hanging-drop 



cultures 80 



Fig. 28. Implements commonly used in plant tissue culture 



work 85 



Fig. 29. Equipment for preparing and transferring cultures 86 



Fig. 30. Procedure of cutting roots within flasks 87 



Fig. 31. Distributing nutrient to flasks 87 



Fig. 32. Method of obtaining sterile adventitious roots 88 



Fig. 33. Excising adventitious roots from cuttings 88 



Fig. 34. Method of obtaining uniform stocks of root tips 113 



Fig. 35. Bean pod segments used in testing wound stimulants 114 



Fig. 36. Eemoving blocks of cambium from large trees 115 



Fig. 37. Procedure in making cultures of cambium 115 



Fig. 38. Cambium culture of Salix caproea 116 



Fig. 39. Surface of cambial cultures of Abies pectinata 127 



Fig. 40. Measuring a root within the culture flask 128 



Fig. 41. Sample pages from a book of records 129 



Fig. 42. Equipment used in making hanging-drop cultures 130 



Fig. 43. Average daily increment rates of 1000 roots during 



one week in vitro 141 



Fig. 44. Effects of temperature on growth rates of tomato 



roots 145 



Fig. 45. Example of a line graph recording daily march of 



increments of excised roots 174 



Fig. 46. Example of a histogram recording effects of nutrient 



variables on growth 175 



Fig. 47. Example of an isopleth diagram showing the effects 



of two simultaneous variables 176 



Fig. 48. Tomato root showing gross effect of indole acetic 



acid on growth 179 



Fig. 49. Effects of iron concentration on root growth 180 



Fig. 50. Effects of iron concentration on root growth 181 



Fig. 51. Detail of the effects of indole acetic acid on cells of 



an excised root 182 



Fig. 52. Graph showing effects of indole acetic acid on growth 



rates 187 



Fig. 53. Graph of rates of water secretion (root pressure) in 



excised tomato roots 191 



