The History of Plant Tissue Culture 21 



and the attempt to attack the problem directly was 

 regretfully abandoned for another decade with the 

 remark that 



' ' Jedenf alls diirf te die Methode ' • At any rate, the method of 



der Ziichtung isolierter Pflan- cultivating isolated plant cells 



zenzellen in Nahrlosungen ver- in nutrient solution should make 



schiedene wichtige Probleme von possible the experimental study 



einer neuen Seite her der ex- of many important problems 



perimentellen Bearbeitung zug- from a new point of view." 



anglich machen." (1902, 98) 



Haberlandt turned to an indirect approach, that 

 of the study of wound healing (1913, 294, 1914, 

 295, 1919, 245, 296, 1920, 297, 1921, 354, 1922, 355), 

 and has never returned to the original problem. 



It was during this unfruitful first decade of the 

 20th century that the sister science of animal tis- 

 sue cultures saw its initiation and greatest devel- 

 opment. Harrison (Fig. 6) in 1907 succeeded in 

 settling an old controversy by just the methods 

 suggested by Haberlandt, when he cultivated the 

 neuroblast of the frog in clotted lymph (1907, 131, 

 1908, 132, 1910, 414). He followed the presenta- 

 tion of his experimental results with a brilliant 

 expose of the possibilities of this method of ap- 

 proach. Two years later Burrows studied with 

 Harrison, subsequently joined Carrel, and to- 

 gether these two established the present-day 

 method of cultivating excised animal tissues in a 

 nutrient made up of blood plasma and embryo 



