Chapter IV 

 THE LABORATORY 



The description of a laboratory given above is 

 presented as an example, not an ideal ! The gen- 

 eral requirements for a plant tissue culture lab- 

 oratory are the same as those for any detailed 

 micro-culture work: 1, facilities for the prepara- 

 tion, sterilization, and storage of nutrients and 

 for the cleansing of used equipment; 2, a place 

 for the aseptic manipulation of tissue masses; 3, 

 facilities for the maintenance of cultures under 

 carefully controlled conditions; 4, facilities for 

 examination and study of cultures in whatever 

 ways may be desired, and 5, a place for the assem- 

 blage and filing of records. It will seldom be 

 possible to carry on all these procedures in a 

 single room with any degree of effectiveness, but 

 the degree of complexity introduced into the or- 

 ganization of the laboratory will depend on the 

 particular needs and the facilities available for 

 any given work. The ideal organization would 

 allow a separate room for each of the above pro- 

 cedures — a media room, a transfer room, one or 

 more culture rooms, a laboratory, and an office. 

 (Compare Parker, 1938, 26.) (Fig. 25.) 



