80 



Plant Tissue Culture 



be handled far more easily than test tubes (which 

 require special sloped racks), they present a 

 larger space in which to work when dissections 

 are to be performed on the cultures, they will 

 stand on an ordinary table without rolling, and 

 they are easily cleaned. They do occupy consid- 

 erable space. Special flasks such as Carrel flasks 



f b C D E 



=c*= 



? : 1 



f.j.r.1 



Zbmn 



Fig. 27. Diagram of a special, pierced slide found particularly 

 satisfactory for hanging-drop cultures of plant tissues. A, tissue 

 fragment. B, drop of nutrient. C, square 22 x 22 mm. No. 1 cover 

 glass on which culture is made. D, small 7x7 mm. No. cover 

 (square or round) held on lower surface of nutrient by capillarity 

 providing a flat lower as well as upper surface to the drop. E, 

 square 22 x 22 mm. No. 2 cover glass closing the bottom of the 

 chamber. 



