112 Plant Tissue Culture 



or highly purified ingredients. These include 

 salts of various kinds (chlorides, nitrates, sulfates, 

 phosphates, and iodides of calcium, magnesium, 

 potassium, sodium, iron, manganese, zinc, and 

 boron) ; carbohydrate (especially sucrose) ; or- 

 ganic nitrogenous materials (amino-acids, tissue 

 digest peptides, peptones, etc.) ; hormones and 

 vitamins (thiamin, nicotinic acid, vitamin B 6 , 

 indole acetic acid, etc.) ; and sometimes coagu- 

 lating agents (agar-agar, gelatine, etc.). 



Standard procedures for the preparation of 

 nutrients are described. 



