102 BINUCLEATE CELLS IN TISSUE CULTURES. 



ous change in outline and appears to be intimately associated with adjacent mito- 

 chondria. 



Vital dyes: Gentian violet did not prove to be a true vital dye. While it 

 stained the intranuclear bodies and nuclear membrane, its action was toxic and 

 coagulative, and the cells speedily degeneratetl. 



Janus green, in low dilutions, was found to stain mitochondria specifically, but 

 its action was destructive, causing speedy death of the cell, with dissolution of the 

 mitochondria. 



Enibryonic alls: Many, at least, of the facts obtaincnl from observation of cells 

 in tissue cultures may be applied to the interpretation of similar cells de\eloping 

 normally in the embryo. 



In conclusion I wish to record my indebtedness to M. K. and \\'. H. Lewis for 

 the loan of their splendid collection of fixed preparations, and for their valued 

 guidance; also to Dr. F. R. Lillie for the courtesy of a room at the Marine Biological 

 Laboratory at Woods Hole, where some of the work was carried on. 



