16 ON THE DIFFERENTIAL REACTION TO VITAL DYES EXHIBITED BY 



Protocol: Mouse 36, October 26, injected subcutaneously with 0.5 c. c. of a 1 per cent 

 solution of isamine blue 6B, a sulphonated triphenyhnethane dye, with the formula 



Nq0 3 5 



ojX)jxrc&* 



x — exact position of sulpbonic radical uncertain. 



November 3: Ears are pale blue; skin over back and sides is deep blue; subcutaneous tissue 

 definite pale blue on side opposite injection-point. 



Injection-site contains many macrophages with typical form and deposits of varying sizes. 

 Fibroblasts arc in the majority well filled with large dye vacuoles, though these same cells may show 

 processes quite free from dye as well as many small vacuoles in parts. 



In less heavily stained parts (transition zone is broad and vague), the cell types are more 

 distinct, though many fibroblasts have large deposits. Here one finds rounded-up macrophages wit h 

 immense vacuoles, while others are packed with paler, comparatively small deposits. The latter, 

 however, are found containing one or many of the larger vacuoles, and similarly those with the largest 

 vacuoles have some smaller also. However, such neighboring cells may be strikingly dissimilar in 

 general appearance. 



The palely stained area shows macrophages with a moderate amount of dye, in the form of 

 small vacuoles, some of which are colorless. The fibroblasts are seen, even under the oil, with more 

 difficulty. They contain often a half dozen or more small round dye granules unrelated to the 

 mitochondrial threads. 



Protocol: Rat 25-1, injected intraperitoncally with a 0.5 per cent solution of the deep-blue 

 " negative" dye H 7, made by combining 1 molecule of dianisidine with 2 molecules of 

 alpha naphthol monosulphonic acid 4, and hence with the formula 



oo» 



OCH 



February 1, 4, 8, 11, 14, 17, 20, 23, 26, March 1, 4, 7, 10, and 13, 2 c. c. each day; March 

 17, 19, 22, and 25, 4 c. c. each day. 



March 27: Skin of scrotum is deep blue ; lower abdominal skin has a pale-violet hue. The animal 

 is otherwise unstained. Under the low-power the skin of the thigh shows macrophages with a very 

 pale blue content of dye. It is only under the oil that one finds the two or three pale-blue deposits 

 that are present in most fibroblasts. Even with neutral red the macrophage reaction is predominent 

 and fibroblasts can not be detected with the low-power and with the oil their vacuoles are lacking 

 in intensity and are scanty in number, but it is certain that the number of the neutral-red bodies is 

 somewhat above normal (fig. 33). Janus green shows normal mitochondria. 



Macrophages of the thigh have a dye-content of a fair number of vacuoles, small and round, 

 of uniform size, in which neutral red shows frequent concretions (fig. 32). In the scrotum these 

 cells are filled with vacuoles of an intense blue color, small, however. At the puncture-point 

 mammoth cells are present, with large vacuoles of deep purple among smaller, more uniform sized 

 vacuoles which are still larger than those found in the thigh (fig. 34). 



In the skin of the scrotum, fibroblasts are distinguished by the enormous number of small 

 round and angular dye bodies which fill the cytoplasm and by the concurrence of cells in which many 

 blue linear forms are present. These threads carry dye concretions at their ends and along their 

 course. Cells of this type occur also at the puncture-point, but are not found more than a few 

 millimeters from it. It is remarkable that they do not occur in the serous membranes, where an 

 enormous number of small deposits crowds the cytoplasm. 



