EXPLANATIONS OF PLATES. 



53 



Fig. 31 



Fig. 32 



Fig. 26. Macrophage. Subcutaneous tissue from thigh of 

 rat which has received 30 daily 1 c. c. intra- 

 peritoneal doses of 2 per cent New Bordeaux L 

 (B.A.S.F.). Examined 36 hours after last in- 

 jection. (See protocol Rat 47, p. 14.) Neutral 

 red 1 : 10,000. 

 Fig. 27. Fibroblast from same case. Neutral red 1:10,000. 

 No marked difference in the size of macrophage 

 and fibroblast vacuoles is apparent. 

 Fig. 28. Macrophage. Subcutaneous tissue from thigh of 

 rat which has received 10 daily 1 c. c. intra- 

 peritoneal doses of 0.5 per cent dye 230, a red 

 diffusible dye made by combining benzidine 

 o-disulphonic acid with 2 molecules of beta 

 naphthylamine 7 monosulphonic acid. Ex- 

 amined 3 days after last injection. (See pro- 

 tocol Rat 94, p. 14.) Neutral red 1 : 10,000. 

 Fig. 29. Fibroblast from same case. Physiological saline. 

 No marked difference in the size of macrophage 

 and fibroblast vacuoles is apparent. 

 Fig. 30. Macrophage. Subcutaneous tissue from thigh of rat 

 which has received sixteen 1 c. c. intraperito- 

 neal doses of 1 per cent 1212 d, a very diffusible 

 bright-red dye made by combining 1 molecule 

 o-tolidine with 2 molecules of beta naphthol 

 8 monosulphonic acid, over a period of 19 days. 

 Examined 1 day after last injection. (See 

 protocol Rat 19, p. 15.) Neutral red 1: 10,000. 

 Fibroblast. Subcutaneous tissue from thigh of 

 eame case. Neutral red 1 : 10,000. No 

 marked difference in the size of macrophage 

 and fibroblast vacuoles is apparent. 



Macrophage. Subcutaneous tissue from thigh of 

 rat which has received fourteen 2 c. c. intra- 

 peritoneal doses, followed by four 4 c. c. doses 

 of 0.5 per cent dye H 7, made by combining 1 

 molecule dianisidine with 2 molecules alpha 

 naphthol monosulphonic acid 4, over a period 

 of 53 days, injected every fourth day. Ex- 

 amined 2 days after last injection. (See pro- 

 tocol Rat 25-1, p. 16.) Neutral red 1 : 10,000. 

 The neutral red brings out emphatio concre- 

 tions. 



Fibroblast from thigh of same case. Neutral red 

 1:10,000. 



Drawing showing size of vacuoles in macrophage 

 from puncture-point area of same case. 



Macrophage. Subcutaneous tissue from thigh of 

 rat which has received 10 daily 1 c. c. doses of 

 0.5 per cent vital new red. Examined 3 days 

 after last injection. (See protocol Rat 10, 

 p. 18.) Physiological saline. 



Another macrophage from same case. Neutral 

 red 1:10,000. 



Fibroblast from same ease. Janus green 1 : 10,000. 

 The pale-green mitochondria are easily sep- 

 arated from the vital-new-red deposits, even 

 though their morphology is strikingly similar. 



Macrophage. Subcutaneous tissue from thigh of 

 rat which has received six 1 c. c. intraperitoneal 

 doses of 0.1 per cent trypan blue over a 

 period of 10 days, injected every fourth day. 

 Examined 1 day after last injection. (See 

 protocol Rat 325, p. 19.) Neutral red 1:5,000. 

 The trypan-blue deposits are seen to be almost 

 exclusively crystalline, although there is some 

 increase of the vacuolar segregation-apparatus 

 as seen by neutral red. 

 Fio. 39. Macrophage. Subcutaneous tissue from abdomen of 

 rat fed Niagara blue BB, the benzidine homo- 

 logue of trypan blue, total 5 to 6 grams, in milk- 

 and-egg mixture over a period of 24 days. (See 

 protocol Rat 4, p. 19). Physiological saline. 

 The scanty vital-dye deposits are usually deep- 

 blue crystalline structures, many of which 

 oan be seen to have a vaouolar origin. 



Fig. 33. 

 Fig. 34. 

 Fio. 35. 



Fig. 36 

 Fig. 37. 



Fio. 38. 



Fig. 40. Macrophage. Subcutaneous tissue from abdomen of 

 rat fed for 13 days on 0.25 per cent dye T 1824 

 (an isomer of trypan blue) solution in milk. 

 Examined at the close of dosage. (See protocol 

 Rat 247, p. 20). Physiological saline. The 

 only vital dye deposits of an appreciable inten- 

 sity are crystalline "sickles." 



Fig. 41. Another macrophage from same case. Physiological 

 saline. 



Fio. 42. Macrophage near blood-vessel. Subcutaneous tissue 

 from thigh of rat which has received 11 

 1 c. c. intraperitoneal doses of 1 per cent 

 solution of dye 245 (an isomer of vital new red) 

 over a period of 27 days. Examined 1 day after 

 last injection. (See protocol Rat 2286, p. 21.) 

 Physiological saline. The "saturation crys- 

 tals," long saffron-colored needles, are con- 

 densing without reference to the mass of 

 vacuoles produced by the dye. 



Fio. 43. Fibroblasts from thigh of same case. Physiological 

 saline. In the majority of cases the vacuolar 

 origin of tho saturation crystal can be ascer- 

 tained. 



Fig. 44. Two contiguous cells, a macrophage and a fibroblast, 

 from the subcutaneous tissue from abdomen 

 of rat which has received four 4 c. c. intra- 

 peritoneal doses of 0.5 per cent dye T 148 over 

 a period of 16 days, injected every fifth day. 

 Examined 40 days after last injection. Neutral 

 red 1 : 10,000. 



Fio. 45. Macrophage from subcutaneous tissue from thigh 

 of same case after treatment with a second dye. 

 The animal received twenty-three 1 c. c. intra- 

 peritoneal doses of 0.5 per cent trypan blue 

 over a period of 58 days. This trypan-blue 

 dosage was started on the day following the 

 above examination (Fig. 44). Examined 1 

 month after last trypan-blue injection. Neutral 

 red 1 : 10,000. The trypan blue by reason of 

 its crystallization has distorted all the previous 

 dye T 148 vacuoles into seed-shaped structures. 



Fia. 46. Fibroblast from same case. Drawn at same date 

 as figure 45. Neutral red 1 : 10,000. The try- 

 pan-blue crystals are with few exceptions 

 forming in the small T 148 vacuoles, though 

 only a portion of the latter are chosen for try- 

 pan deposits. 



Fio. 47. Macrophage. Subcutaneous tissue from thigh of 

 rat which has received 43 daily 1 c. c. intra- 

 peritoneal doses of 0.1 per cent dye T 148, 

 followed immediately by 6 daily 1 c. c. doses 

 of 0.5 per cent trypan blue. Examined 15 

 hours after last injection. (See protocol Rat 

 53, p. 23.) Neutral red 1:10,000. Crystals 

 of trypan blue are seen condensing in the 

 peripheral portion of the vacuoles, i. e., at the 

 vacuole-protoplasm interface. 



Fig. 48. Another macrophage from same case. Neutral red 

 1 : 10,000. 



Fig. 49. Fibroblast. Subcutaneous tissue from thigh of rat 

 which has received twenty 1 c. c. intraperito- 

 neal doses of 0.5 per cent trypan blue over a 

 period of 57 days. Examined 2 days after 

 last injection. (See protocol Rat 16, p. 25.) 

 Neutral red 1: 10,000. Many deop-blue crys- 

 talline deposits of the vital dye are seen and, 

 in addition, a considerable filar segregation- 

 apparatus stained electively with neutral red. 



Fig. 50. Macrophage from same case. Neutral red 

 1: 10,000. The cell is the seat of many crys- 

 talline vital-dye deposits. Many of the crys- 

 tals are no longer associated with vacuoles. 



