54 



EXPLANATIONS OF PLATES. 



Fie. 61. Macrophage from same rase after the animal has 

 received 10 daily 1 c. c. intraperitoneal doses 

 of 0.1 per cent dye T 148. Examined on the 

 day following the last injection. Neutral 

 red 1:10,000. Almost all of the crystalline 

 trypan-hlue deposits are now inclosed by 

 vacuoles produced by the subsequent treatment 

 with the red dye. 



Fio. 62. Fibroblast from subcutaneous tissue of thigh from 

 samo case, also after dye T 148 dosage. 

 Neutral red 1 : 10,000. Only a single instance 

 of the inclosure of a trypan-blue crystal within 

 a T 148 vacuole can be seen; the majority of 

 these dense-blue fibroblastic deposits are 

 unaffected by tho extensive vacuolar Begrega- 

 tion-apparatus. 



Fio. 63. Fibroblast. Subcutaneous tissue from thigh of rat 

 which has received fifteen 1 c. c. intraperitoneal 

 doses of 0.1 per cent trypan blue over a period 

 of 42 days (injected every fourth day). Ex- 

 amined 3 days after last injection. (See 

 protocol Rat 43, p. 27.) 



Fio 64- Macrophage from subcutaneous tissue from the 

 abdomen of same animal after it had received 

 1 1 daily 1 c. c. intraperitoneal doses of 0.5 per 

 cent dye T 148 immediately subsequent to the 

 trypan-blue dosage. Examined 2 days after 

 last injection. Physiological saline. The pink 

 vacuoles (the larger ones are uncolored) are 

 produced by the red dye and have almost 

 completely dissolved the blue deposits. 



Fio. 65. Fibroblast from same case, examined after the 

 above dye T 148 dosage. Neutral red 1 : 10,000. 

 The majority of the blue crystalline trypan 

 deposits are not affected by the treatment 

 with the red dye (dye T 148). 



Fio. 60. Another fibroblast from the samo case, examined 

 after the above dye T 148 dosage. Neutral red 

 1 : 10,000. Here most of the trypan-blue 

 crystals are seen to be inclosed in the elaborate 

 vacuolar segregation-apparatus produced by 

 the subsequent red dye. 



Fio. 67. Another macrophage from the same case, examined 

 after the dye T 148 dosage. Neutral red 

 1:10,000. Here tho seed-like and pure crys- 

 talline trypan-blue deposits are as yet but 

 little affected by the secondary dosage with 

 the red dye (dye T 148). 



Fio. 68. Macrophage. Subcutaneous tissue from thigh of 

 rat which has received twelve 1 o. o. intra- 

 peritoneal doses of 0.5 per cent trypan blue over 

 a period of 44 days. Examined 3 days after 

 last injection. (See protocol Rat 15, p. 28.) 

 Neutral red 1 : 10,000. This is a typical pic- 

 ture of the angular distortion of the vacuolar 

 segregation-apparatus caused by extensive 

 crystal production. It is somewhat more en- 

 gorged with trypan blue than is usual in this 

 case. 



Fio. 60. Fibroblast from thigh of same case. Neutral red 

 1 : 10,000. A very typical picture of the 

 crystalline deposition of trypan blue in fibro- 

 blasts; part of the segregation-apparatus waB so 

 faint in color that neutral red waB needed to 

 show itB complete extent. 



Fio. 60. Macrophage from subcutaneous tissue of samo rat 

 after it had received one 4 c. c. intraperitoneal 

 dose of 1 per cent dye T 148 and one 2 c. o. 

 dose of the eame on the fifth and seventh days 

 respectively after tho trypan blue waB dis- 

 continued. Examined 2 days after last in- 

 jection of dye T 148. Neutral red 1: 10,000. 

 Most of the trypan-blue crystalline deposits 

 are inclosed in the secondary segregation- 

 apparatus oreated by the administration of 

 the red dye (dye T 148). 



Fio. 01 



Fio. 62. 



Fio. 63 



Fio. 64, 

 Fio. 65. 

 Fio. 66. 

 Fio. 07. 



Fio. 68. 

 Fio. 09. 



Fio. 70. 



Fio. 71. 



Fibroblast. Subcutaneous tissue from thigh of 

 same case after the dyo T 148 dosage. Physi- 

 ological saline. It is surprising how few of the 

 crystalline trypan-blue fibroblast deposits have 

 been affected by the subsequent dye treatment 

 with dye T 148. 



Macrophage. Subcutaneous tissue from thigh of 

 rat which has received 8 daily 1 c. o. intra- 

 peritoneal doses of 0.5 per cent trypan blue. 

 Examined 13 days after last injection. (See 

 protocol Rat 222, p. 28.) Neutral red 

 1:10,000. Tho tendency has been to ob- 

 literate the usual appcuranco of the vacuolar 

 segregation-apparatus altogether and to sub- 

 stitute substantial spear-shaped crystalline 

 accumulations. 



Macrophage from tho same case examined 2 days 

 after an intraperitoneal injection of 4 o. o. of 

 1 per cent dye T 148 (immediately following 

 the above trypan-blue doaage). Physiological 

 saline. In profound contrast to the condition 

 preceding treatment with the red dye T 148 

 (fig. 62); the large vacuolar segregation- 

 apparatus created by T 148 has almost invari- 

 ably inclosed the deposits of crystalline blue. 



Fibroblast from subcutaneous tissues of abdomen 

 from eame case, after the dye T 148 dosage. 

 Physiological saline. 



Mitochondria of a fibroblast. Subcutaneous tissue 

 from thigh of a normal rat. Janus green 

 1:10,000. 



Mitochondria of a fibroblast. Subcutaneous tissue 

 from thigh of a normal mouse. Janus green 

 1:10,000. 



Fibroblast. Subcutaneous tissue from thigh of rat 

 which has received 13 daily 1 c. o. intraperito- 

 neal doses of 0.6 per cent dyo 227, a brownish- 

 red diffusible dye made by combining 1 mole- 

 cule of benzidine-disulphonic acid with 2 

 molecules 2 amido 8 naphthal 6 monosul- 

 phonic acid, followed by 6even 1 o. o. doses 

 given every other day. Examined 1 day after 

 last injection. Janus green 1: 10,000. The blue- 

 green mitochondria are in sharp contrast to 

 tho red vital -dye deposits. 

 Macrophage from samo case. Neutral red 1:10.000. 



Fibroblast. Subcutaneous tissue fiom thigh of rat 

 which has received daily 1 c. o. intraperitoneal 

 doses of 0.5 per cent dye T 257 (a dye made by 

 combining 1 molecule of o-tolidine with 2 

 molecules beta naphthylamine 5.7 disulphonio 

 acid. Examined 1 day after last injection. 

 Janus green 1:10,000. The blue-green mito- 

 chondria are in conspicuous contrast to the 

 pale pink segregation-apparatus oreated by the 

 vital azo dye. Some filar distortions of the 

 vacuoles are seen. 



Fibroblast. Subcutaneous tissue of mouse which 

 has received nino 0.5 c. c. intraperitoneal 

 doses of 0.5 per cent trypan blue over a period 

 of 63 days, followed after a rest of 24 days by 

 0.2 c. c. of 2 per cent T 148. Examined 12 

 days after the last injection. Janus green 

 1:10,000. The mitochondria occupy a rather 

 distinct protoplasmic lone. 



Fibroblast. Subcutaneous tissue from thigh of rat 

 which has received four 4 o. o. intraperitoneal 

 dot>es of 0.5 per cent T 148 over a period of 10 

 days (injected every sixth day). Examined 

 1 day after last injection. Janus greea 

 1:5,000 (half-minute exposure). The deep 

 blue-green mitochondria are in striking con- 

 trast to the pale-pink vacuolar segregation- 

 apparatus produced by the vital dye; filar 

 distortions of the vacuoles are evident at 

 several plaoes. 



