EXPLANATIONS OF PLATES. 



55 



Fio. 72. Fibroblast. Suhcutaneous tissue from back of rat 

 which has received nine 1. e. c. suboutaneoua 

 injections of 1.5 per cent isamine blue over a 

 period of 28 days (injected twice a week). 

 Examined 1 1 \i months after last injection. 

 (See protocol Rat 200, p. 33.) Neutral red 

 1 : 10,000. The cell is from the actual puncture 

 area and shows strikingly how in such situa- 

 tions even a negative dye may have oppor- 

 tunity to completely fill the fibroblastic cyto- 

 plasm with an elaborate vacuolar segregation- 

 apparatus. 



Fio. 73. Small area of the areolar tisBue of the thigh of a rat 

 which has received 18 1 c. c. intraperitoneal 

 doses of 1 per cent trypan blue over a period 

 of 61 days (injected every fourth day). Ex- 

 amined 2 days after last injection. (See pro- 

 tocol Rat 46, p. 34.) Practically all of the 

 deposits in both types of cell are crystalline. 

 Physiological saline. X 160. 



Fig. 74. Fibroblast from same case. Neutral red 1:10,000. 

 This beautiful and typical picture shows the 

 long, delicate diamond and spear shaped crys- 

 talline vital-dye deposits, which are here al- 

 most the exclusive form of the deposit in the 

 fibroblasts. 



Fio. 75. Macrophages from the same case. Physiological 

 saline. 



Fio. 76. Macrophage and fibroblast. Subcutaneous tissue 

 from abdomen of rat which has received nine 

 1 c. c. intraperitoneal doses of 1 per cent trypan 

 blue over a period of 24 days (injected every 

 fourth day). Examined 1 day after the laBt 

 injection. Physiological saline. The deposits 

 are less thread-like here than is the case in 

 fibroblasts from remoter areas. 



Fio. 77. Fibroblast. Subcutaneous tissue from thigh of rat 

 which has received 10 daily 1 c. c. intraperito- 

 neal doses of 0.5 per cent dye 226, a bright-red 

 dye made by combining 1 molecule benzidine 

 metadisulphonic acid with 2 molecules of the 1.8 

 amido naphthal 4.6 disulphonic and the so-called 

 K acid. Neutral red 1:10,000. The neutral 

 red, a supravital stain which was necessary to 

 bring out the complete extent of the segrega- 

 tion apparatus and to show occasional filar 

 modifications of the same, haa made the 

 amethyst crystals of the vital acid dye a deep 

 red. 



Fio. 78. Fibroblast. Subcutaneous tissue from opposite 

 thigh of same animal after it had received 9 

 more 1 c. c. injections of the same solution (over 

 a period of 15 days). Examined 10 days after 

 last injection. Neutral red 1 : 10,000. 



Fio. 79. Macrophage from the same case (drawn at the same 

 time as fibroblast, figure 78). Neutral red 

 1:10,000. 



Fio. 80. Fibroblast. Subcutaneous tissue from abdomen of 

 same rat after it had received a total of fifty-one 

 1 c. c. intraperitoneal doses of 0.5 per cent 

 dye 226 over a period of 122 dayB. Examined 

 1 day after last injection. Physiological saline. 

 The various forms and the amethyst color 

 of the crystalline deposits of the vital dye are 

 well shown. 



Fio. 81. Macrophage. Subcutaneous tissue from thigh of 

 rat which has received 8 daily 1 c. c. intraperi- 

 toneal doses of 2 per cent trisulfonazoblau (a 

 deep-purple dye made by combining 1 molecule 

 of benzidine with 2 molecules of alpha naphthol 

 3.6.8 trisulphonic acid). Examined 4 days after 

 the last injection. Neutral red 1 : 10,000. The 

 olive-green rhomboidal crystalline deposits 

 of the vital dye are dispersed among the abun- 

 dant vacuoles, in which the dye is pale pink 

 in color and in part of which a minute deep- 

 purple concrement formation has ensued. 



Fio. 82. Fibroblast. Subcutaneous tissue from rat which has 

 received 26 daily 1 c. c. intraperitoneal doses 

 of 0.5 per cent dye T 148. Examined 1 day 

 after last injection. (See protocol Rat 18-1, 

 p. 36.) Physiological saline. Extensive vacuo- 

 lar segregation-apparatus; some crystalline 

 condensation. 



Fio. 83. Macrophage from same case. Neutral red 1: 10,000. 

 The vacuoles which house the vital dye are not 

 greater than those characterizing the fibro- 

 blasts. 



Fio. 84. Fibroblast. Subcutaneous tissue from thigh of rat 

 which has received ten 0.5 c c. and two 4 c. c. 

 intraperitoneal doses of 1 per cent vital new red 

 over a period of 66 days. Examined 8 months 

 after the last dose. (See protocol Rat 312, 

 p. 39.) Neutral red 1:10,000. The neutral 

 red demonstrates that a segregation-apparatus 

 other than that housing the deep-red perma- 

 nent concretions of vital new red does not exist. 



Fio. 85. Macrophage from the same case. Neutral red 

 1 : 10,000. The Blight content in vital-new-red 

 concretions when compared with fibroblasts 

 (fig. 84) is remarkable. Tho neutral red shows 

 an extensive segregation-apparatus which has 

 lost its vital azo dye. 



Fio. 86. Fibroblast. Subcutaneous tissue from thigh of rat 

 which has received 10 daily 1 c. c. intraperi- 

 toneal doses of 0.5 per cent dye 230. Examined 

 3 days after last injection. Janus gieen 

 1 : 10,000. The mitochondria were partially 

 degenerated at time of drawing. 



Fio. 87. Fibroblast. Subcutaneous tissue from thigh of rat 

 which has received 4 daily 4 c. c. intraperito- 

 neal doses of 1 per cent and 2 of 0.5 per cent 

 trypan blue. Examined 1 day after last 

 injection. Neutral red 1:10,000. The case 

 shows well the tendency of this dye to establish 

 filar modifications of the segregation-apparatus. 



