FIELDS G-l and G-2 

 Columns 27 and 28 



1 5. Indications that an extirpated test organ or tissue is whole (Symbol R) vs. the indication 

 that the test organ or tissue has been macerated, sliced, etc. (Symbols U, V, W, and X) 



Symbol R is used to code the isolation of a whole organ (e. g. , the heart), a representative 

 section of tissue (e. g. , liver tissue), a cell (e. g. , a neuron), or a representative sample of fluid 

 (e. g. , whole blood), which is subsequently treated. Symbols U, V, W, and X, on the other hand, 

 are used to describe specific experimental preparations following any isolation. For example, an 

 isolated tissue might be macerated to a suspension (Symbol U), filtered for the extract (Symbol V), 

 or subsequently grown on culture media (Symbol W), or micro- sliced (Symbol X). 



The manipulation of the tissues, implied by Symbols U, V, W, and X, represents the beginning 

 of a preparation which may be preceded or followed, after some time interval, by introduction of the 

 test compound. Time intervals such as this are ordinarily coded in Field R. For example, the time 

 interval between (a) preparation of a TISSUE (Symbol U, V, W, or X in Field G) for an enzyme study 

 and (b) the introduction of the test compound may be significant and, if given, warrants recording in 

 Field R. 



16. Symbols U, V, W, and X describe states of the test organism, organ, or tissue at the time 

 of the chemical treatment 



When a compound is applied to an organism or organ which is later homogenized or made into 

 slices or extracts, etc. , for purpose of reading the test results, Code Symbols U, V, W, and X can 

 not be coded in Field G. This field is used only to describe the state or condition of the test organism 

 at the time of the treatment. 



17. Symbol Z (Field G-2 only) 



This symbol is used in a unique way and does not correspond to the other symbols of Field G 

 in that it does not represent just a state of the organism during the test, but it represents, like the 

 test compound, an active factor of the treatment. When this symbol is used, it indicates a treatment 

 used on the test organism (which may be in any experimental state indicated by any of Symbols 1 

 through X), in conjunction with the test compound treatment (or test compound and secondary compound 

 treatment). (See the first division [last paragraph] of the section on General Use of Field G. ) Symbol 

 Z is coded only in Field G-2, for sake of consistency, and must be coded in any such test which in- 

 cludes non-chemical treatment as defined in the Code. Symbol Z takes priority over Symbols 1 through 

 X in Field G-2. 



18. Symbols available for additional items of Field G 



All numerical and letter symbols available for Field G have been used, with the exception of 

 Symbol Y. 



19. File of coded biology data on IBM punched cards arranged according to symbols for 

 experimental states 



The CBCC has established no special file of coded data in which Field G has been used (and 

 arranged by Field G symbol sequence), because of the remote probabilities of a frequent need to search 

 for all information on tests in which was a specific incidental condition of the test organism. 



20. Double coding is not possible in Fields G-l and G-2 



Having made use of all of numbers 1 through 9 and all 26 letters for code symbols in Field G, 

 the IBM machine punching and retrieval procedures do not permit more than a single symbol in Field G 

 in any one line. Therefore, when coding two or more tests whose details and outcomes are so nearly 

 alike that the code lines for all tests would be identical except for differences in pretreatment or state 

 of the test organism (Field G), the tests can not all be recorded by one code line with several entries 

 (representing the several pretreatments or states) in a single column (either Field G-l or Field G-2). 

 (A special circumstance allows condensing two or more tests in a single line by use of Symbol F of 

 Field G [explained in Division 9], but this is not strictly comparable to double coding as it is described 

 in the preceding sentence and even in this special case, Field G is occupied with but a single symbol. ) 



- 57 - 



