FIELD J 

 Columns 37, 38, 39, 

 40, 41, and 42 



The 1 1 or zone punch in Fields M and N, then, is interpreted as indicating that, in spite of 

 an entry in Field J, the dose coded is the dose administered directly to the test organism or an organ 

 of the test organism of Field E or that it is the concentration of the test compound in the host to which 

 the test organism is exposed, as determined by analysis of the host some time after administration to 

 the host. In other words, the 1 1 or zone punch indicates that the coded dose is not the dose 

 (concentration or quantity) administered to the host coded in Field J. (This is explained also in 

 Fields M and N, Specific Directions and Explanations section, Divisions 5, 6, and 8. ) 



9. Relationship between Field J and the anatomy fields, H-l, H-2, and I 



When Field J is coded with a host organism , Fields H-l, H-2, and I are used only to code 

 organs and tissues of that host organism . However, when Field J is coded with a non-living host 

 (a nutrient medium, bath, etc. ), the anatomy fields are used only for coding parts of organs and 

 tissues of the test organism . 



Symbols for non-living hosts all begin with letters recorded on the IBM punched card by using 

 the zone punch (letters S through Z). Therefore, when Field J is coded with a symbol beginning with 

 any of letters S through Z (i. e. , if it is punched with the zone punch in Column 37), any entry in 

 Field H-l, H-2, or I represents anatomical parts of the test organism in Field E. When Field J is 

 coded with a symbol beginning with any number or any letter A through R (i. e. , if it is not punched 

 with the zone punch in Column 37), any entry in Field H-l, H-2, or I represents anatomical parts 

 of the host organism in Field J. 



10. Relationship between Field J and Fields K and L 



Rather than use a single coding area for coding special experimental conditions and sex and 

 stage of the test organism (when no host organism is involved in the test) or of the host organism 

 (when a host_is involved), the CBCC has found it necessary to have two such areas. Thus, Fields F 

 and G represent the coding area used only for sex and stage and special experimental conditions of the 

 test organism , whether or not the test organism is in a host. The two fields, K and L, describe the 

 sex and stage and special experimental condition of any host and if no host is coded, Fields K and L 

 are unused. 



1 1. Variations of standard nutrient media and solutions 



Among the non-living hosts are listed a number of standard nutrient media or saline solutions 

 (e. g. , Ringer's solution and Tyrode's solution). Occasionally, one of these will be altered slightly 

 for some experimental reason and will be reported thus (e. g. , "potassium-free Tyrode's solution"). 

 Instead of assigning a unique symbol to each of these many variants of a standard medium or solution, 

 the CBCC uses the symbol for the standard solution and explains the deviation from the standard on 

 the Code Sheet in the written abstract portion for Field J. 



12. Definition of "culture medium"; use and significance of symbols of the X series 



Symbols of the X series (of the group of non-living hosts, S through Z ) are defined 



as "media" and, as such, refer to laboratory- prepared mixtures of known composition which have been 

 determined to be adequate for satisfying the test organism's requirements for life, growth, and repro- 

 duction. It is possible that a completely adequate culture medium may consist merely of one of the 



specific natural materials coded by Symbols S through W and Y and Z . For example, a 



sugar solution (Symbol T6) or cheese (Symbol W51) may be entirely adequate as a culture medium for 

 growth of an organism (e. g. , a given mold) to be tested for response to a test compound; the natural 

 materials (e. g. , yeast concentrates, eggs, flour, cheese, etc. ), however, lack the degree of 

 standardization that is possible with artificially prepared mixtures. 



The symbols of series X in Field J are not necessarily conceived as making distinction 



between inadequacy and adequacy of a non-living host (i. e. , while items coded with symbols of 



series X are assumed to be adequate for the test organism, items coded with other symbols are 



not assumed to be inadequate for the organism being grown in or on it), nor to make an absolute dis- 

 tinction between the artificiality or naturalness of the non-living host, nor between the standardization 

 or non- standardization of the non-living host. Symbols of the X series pretend a function no broader 



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