FIELDS M and N 

 Columns 45 and 46; 

 47 and 48 



connected to the parent: The dosage applied to the parent is recorded in Fields M and N and the fact 

 that the response is read on the embryo or offspring (coded in Fields E and F) is indicated by Symbol 

 in Field S-3. 



5. Relationship between the dosage fields (Fields M and N) and Fields H and I; use of Symbol # 

 when Field J is NOT coded. (See Divisions 6, 8, and 9 for the use of zone punches when 

 Field J IS coded. ) 



Occasionally, data are encountered in which the test compound was applied to a test organism 

 to determine the effect on some component organ or cell of the test organism and the author has deter- 

 mined the actual concentration of the test compound, at the site of action within the organism, to 

 which that organ or cell is exposed. For example, a 1% solution is administered to a test organism in 

 a volume calculated to give a final dose of 10 mg/kg. An analysis revealed a blood level of 0. 1 mg/cc. 

 The action was on leucocytes. In this case, since the action is stipulated as being local and at the 

 organ where the 0. 1 mg/cc concentration existed, the dosage to be coded is the 0. 1 mg/cc. The dosage 

 administered to the gross organism (1% solution to give 10 mg/kg) is not coded, though it should be 

 included in the written abstract as a record of the technique that gave the 0. 1 mg/cc blood level. 



In such cases, the administration must no longer be regarded as being to the test organism 

 (Field E) whose component was affected. Instead, it is the concentration to which the test organism 

 component, the organ (Field H) or tissue (Field I), is exposed. For this reason, the IBM 11 zone punch 

 (Code Symbol #) is used in the dosage fields, Columns 46 and/or 48, to give this special meaning to 

 the dosage entry. Thus, when Field J is not coded and the Fields M and/or N are coded with Symbol #, 

 the dosage coded is interpreted as the dose applied to the organ or tissue in Fields H or I and is not 

 the dose applied to the gross test organism in Field E. (See the diagram of Division 9. ) 



6. Significance of the relationship between the coding in the dosage fields (Fields M and N) 

 and Field J; use of Symbol # (IBM 11 zone punch) when Field J is coded 



In nearly all cases when Field J is coded, administration of the test compound to that host or 

 host environment is implied and it is assumed that the organism, pathology, or tumor in Field E 

 received the test compound only to the same degree as any neighboring part of its host or host envi- 

 ronment. In those more rare instances, when Field J is coded with a host or host environment, yet 

 (1) application is actually directly to a test organism or tumor or pathological organ so that influence 

 of the dosage expressed is fully upon the test organism, tumor, or diseased organ or (2) the author 

 has determined the final concentration of the test compound in the host or host environment (although 

 application is directly to the host or host environment ) so that the dosage expressed is the actual 

 concentration to which the organism, pathology, or tumor in Field E is exposed, a coding expedient 

 has been resorted to for indicating it. The IBM 11 zone punch, coded by the symbol #, is used in 

 Fields M and N, Columns 46 and 48, to indicate that the coded dose was not the dose administered 

 to the host coded in Field J but was the dose to which the organism, tumor, or disease coded in 

 Field E was directly exposed, regardless of the actual route of administration indicated in Field S-3. 



Symbol # is used in Fields M and N when a host is coded in Field J and-- 



(1) the application is to the test organism and the dosage is expressed as a concentration 

 or quantity to which the test organism is exposed, although the test organism is 

 attached to a host coded in Field J, or-- 



(2) the application is to the host but the dosage expressed is the concentration to which 

 the test organism (Field E) is exposed. 



Symbol # is no_t_used in Fields M and N when a host is coded in Field J and the application is 

 to the host in terms of quantity per unit of the host (e. g. , mgAg- gal/acre, etc. ). Although this is 

 admittedly an expression of the concentration to which the test organism is exposed (as in situation 2 

 above), the terms of the quantity-per-unit-of-organism-or-environment scales are applicable to the 

 host . (I. e. , when administration is to the host or host environment, the terms of the scales represent 

 quantity per unit of host or host environment , not quantity per unit of test organism. ) Symbol # would 

 only be used with these scales (under circumstances of Field J being coded) if the application were to 

 the test organism and the dosage was therefore in terms of quantity per unit of the test organism (as 

 in situation 1 above). See Division 5 above and Division 9 for the use of Symbol # when Field J is 

 not coded. 



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