REACTION OF CELLS 



65 



in a given time, e.g. 10 minutes. If, for instance, we 

 find that the fourfold quantity is necessary for 

 reaching the same haemolytic effect at 29 C. as at 

 39° C, we may say that — according to the ^-rule — 

 the same quantity of ammonia would occupy a time 

 four times as long to produce the same effect at 29° 

 C. as at 39 C. 



Such determinations have been carried out on a 

 very large scale with different haemolytic agents by 

 Madsen and his co-workers Walbum and Noguchi. 

 As an instance, I give a series for ammonia with a 

 time of action of 10 minutes, t is the temperature, q 

 the necessary quantity in cc. of a 0-5 normal NH 3 

 solution. The total quantity was 8 cc. containing 1 

 per cent of red blood -corpuscles from a horse. 

 ^ obs> is the observed, ^ calc#> a calculated quantity 

 evaluated by means of the general formula for the 

 influence of temperature on the velocity of reactions. 

 The degree of haemolysis was 1 7 per cent. 



Haemolysis by means of Ammonia at different 



Temperatures 



The value of fi used for the calculation, which 

 agrees very well with the observation, is 26,760. 



