IX. ELECTROPHORESIS 



283 



the several requirements outlined above. That shown in Figure 1 is 

 the Abramson cell* {5,6) and is constructed of Pyrex glass, the flat 

 portion being made of polished Pyrex slides 0.6 mm. in thickness and 

 spaced 0.6 mm. apart. The cellf in Figure 2 is one devised by Briggs 

 (7) and is also constructed of Pyrex, the flat portion {A) being made 

 of polished Pyrex slides 1.0 mm. thick and spaced 0.8 mm. apart. 

 The Abramson cell employs Cu-CuS04 electrodes. These are 

 connected to the flat portion of the cell through the lumens of right 

 angle bore stopcocks, which can be turned to other positions to allow 

 filling of the cell with the suspension to be studied. Care must be 

 taken to wash fluid through the stopcock lumens in such a direction 

 as to carr}^ away any electrolyte that may have diffused into them 

 during preceding measurements, before fresh suspension is allowed 

 to pass into the flat cell. 



Fig. 2. Flat horizontal microelectrophoresis 

 cell of Briggs. 



The Briggs cell uses Hg-HgNOs-KNOg electrodes {B, Fig. 2), 

 which can be removed easily and cleaned and are connected to the 

 cell proper by standard taper, ground glass joints. This cell is so 

 constructed that fresh fluid containing the particles can be introduced 

 at any time (through D) without danger of contamination by elec- 

 trode electrolytes since the movement of this fluid will always be past 

 the electrode connection in a direction away from the flat part of the 

 cell. Flow of the test fluid past each electrode is governed by opening 

 or closing stopcocks (C) at the ends of the cell, and the outlets are so 

 arranged that, once the cell is filled, opening the outlet stopcocks can- 

 not result in complete evacuation of liquid from the cell proper. 



* Obtainable from Hopf Glass Apparatus Co., 192 Third Ave., New York 

 City. 



t This cell may be obtained from the University Glass Blowing Shop, Uni- 

 versity of Minnesota, Minneapolis, Minnesota. 



