76 



KARL FOLKERS 



TABLE IV 

 Fermentation "L. Casei" Factor and Vitamin Be Conjugate 



Fermentation "L. casei" Factor 

 Isolation (1944) 

 Hutchings and coworkers 



O 



N N 



N OH 



N 



R =: three glutamic acid moieties 



Vitamin Be Conjugate 

 Isolation (1945) 

 PfifTner and coworkers 



O 

 II 



R'C< 



N N 



^NHCH, 



N OH 



NH, 



N 



R'zz: seven glutamic acid moieties 



There are still other factors which may be related to pteroylglutamic 

 acid. 



In their attempts to prepare a concentrate of folic acid from by- 

 products of commercial liver extract, Barton-Wright, Emery, and Rob- 

 inson found that there were chloroform-soluble fractions which were 

 active for the growth of L. helveticus and ^. lactis. 



Daft and Sebrell have studied the anemia and granulocytopenia 

 which develop in rats on a pantothenic acid-deficient diet but with added 

 L. casei factor. Treatment with whole dried liver was more effective 

 than treatment with pantothenic acid. A factor or factors in the liver 

 were indicated. 



The significance of these observations and a possible relationship to 

 pteroylglutamic acid is not yet clear (Table VII). 



Because of abundant evidence on the antianemic properties of pteroyl- 

 glutamic acid, or vitamin Be, on experimental animals, it is not sur- 

 prising that pteroylglutamic acid showed antianemic properties in man. 



