UNIDENTIFIED VITAMINS 83 



TABLE XIII 



Isolation of a New Leukopoietic Factor from Liver (Cline, Berry, and 



Spies, 1945) 



Liver extract acetone acid-washed acetone 



> . > > crystals 



(reticulogen) fractionation permutit water fractionation 



Properties of Crystals 



1. positive test primary aliphatic amino group 



2. contained "ash" 



3. 20-60 mg. dose for malnourished patients with leukopenia caused in- 

 crease in white cell count and neutrophils 



tissue which resulted from changing conditions in the animal as a 

 whole. These investigations resulted in the discovery that raw cream 

 contains a factor essential for the guinea pig which differs from those 

 factors previously described for the guinea pig. Deficiency of this 

 factor caused a stiffness of the wrists and elbows of the animals and 

 calcium triphosphate depositions throughout the whole body (Table 

 XIV). 



TABLE XIV 



A Dietary Factor Essential for Guinea Pigs, AntistiflFness Factor 



Growth and disease of planarian worms fed tissues of guinea pigs (Wulzen 

 and Bahrs, 1936-1941). 



Raw cream contains factor for guinea pigs. 



Deficiency characterized by stiffness in wrists and elbows and calcium 

 triphosphate depositions. 



Partial isolation of factor from raw cream (van Wagtendonk and Wulzen, 

 1943)- 



A report on the partial isolation of this factor from raw cream was 

 made in 1943 by van Wagtendonk and Wulzen. 



It was found that a deficiency of the antistiffness factor had no effect 

 upon the creatine excretion. In contrast with this result, vitamin E 

 deficiency produced muscular dystrophy accompanied by creatinurea. 



The concentration of the easily hydrolyzable phosphorus fraction 



