UNIDENTIFIED VITAMINS 85 



TABLE XVI 

 Sources of the Antistiffness Factor 



55 gal. raw cream >3 mg. concentrate, curative at o.iy dosage 



Activity of Source Materials (van Wagtendonk and Wulzen, 1946) 



Material Activity, units/mg. 



Raw cream ' i 



Raw potato 0.5 



Baker's yeast o 



Beef muscle and liver 0.5 



Alfalfa 0.5 



Broccoli O 



Soy bean o 

 Beet molasses o 



Cane molasses 10 

 Cane juice 100- 1000 



activity at the levels tested. Cane molasses and cane juice were discovered 

 to be excellent sources of the antistiffness factor and isolation work 

 was started with these raw materials. 



A crystalline compound was eventually isolated from crude cane 

 juice. The process for the isolation of the crystalline compound is 

 shown schematically in Table XVII. The crude cane juice showed an 

 activity of 100 u./g. It was first extracted with ether. Distillation of 

 the ether left a residue which showed one million u./g. Distribution of 



TABLE XVII 



Isolation of the Antistiffness Factor from Cane Juice (van Wagtendonk 



and Wulzen, 1946) 



Crude cane juice ether f extract ~| distillation residue distribution 



(55 gal.) > \ residual \ ^ (los u./g.) ^ 



(100 u./g.) extraction [ solution J pet. ether and 



90% menthanol 



activity in distillation residue MgO MgO adsorbate distillation 



pet. ether ^ (1.5 x lo^ u./g.) > solution > 



solution pet. ether 



and benzene 



residue molecular sublimations crystalline material 



(10 X iQfi u./g.) > m.p. 81.5-82° 



and recrystallizations activity, 500 x 10^ u./g. 



yield, 100 mg. 



dosage, 0.0027 



