

f^^^ 







* ** 



Plate II. Induction of cartilage formation by dead cartilage graft in Amblystonia 

 tigrinum (X 185). A metatarsal bone, quick-frozen in isopentane at — i59°C, debydrated 

 in vacuo at — 40°C, reliydrated in Ringer's solution, and transplanted into the tarsus of 

 another premetamorphic specimen of the same species, has healed in; new cartilage and a 

 new perichondrium have formed along most of the surface of the epiphysis of the graft, 

 which has become detached from the bony shaft, seen in the right half of the picture. The 

 new cartilage can be clearly distinguished by its darkly stained nuclei from the dead graft 

 with unstained ghosts of dead nuclei in its capsules. 



