BY R. GREIG-SMITH. 



91 



to see how far this was justified. Wide-mouthed ounce bottles 

 were used, and each received 2 c.c. of the Eucalyptus oil, which 

 was sufficient to cover the dome-shaped bottom of the bottle. 

 The bottles were then heated in the incubator at 37° for half-an- 

 hour to get them warmed up, aird so ensure the temperature 

 being constant during the test. Threads of cotton, attached to 

 wires similar to those used in the tests with olive oil, were im- 

 pregnated with loops of a 20-hour broth-culture of the test 

 organism. Each received the charge of one loop made by twist- 

 ing a thin wire around a thicker wire(B. W.G. 12). The infected 

 thread was suspended in the middle of the air-space of the bottle, 

 the wire being held in place by the cotton plug. During the 

 test, the bottles were kept in the incubator at 37°. 



A few tests were made with the ordinary oils, but, as there 

 was the possibility that the acid in them might have some influ- 

 ence upon the bactericidal power, three of the oils were treated 

 with lime before testing. For the sake of simplicity, all the 

 tests are grouped in one Table. The time when the bacteria 

 were destroyed and the time just short of this are given in order 

 to show the spacing between the times in each case. Growth is 

 indicated in the usual manner by a " + " and absence of growth 

 by a "O" in the headings of the columns. 



Table ix. — Germicidal Effect of the vapour. 



The vapours of the oils have an undoubted disinfecting action 



