BY R. QREIG-SMITH. 



337 



The new culture was almost twice as sensitive to the action 

 of the disinfectant as the stock culture with which the work had 

 been done. 



The result of the test led to the trying of nine colonies, picked 

 out at random from a plate containing about fifty colonies, and, 

 in order to economise labour and material, the time test with 

 single dilutions was employed. A spiral loop of broth culture 

 (7 milligrams; was put into 2 c.c. of diluted disinfectant and 

 portions were withdrawn at intervals of 5, 10, 15, and 30 minutes. 

 Longer exposures were all negative. 



Tablk xvii. Minutes required to destroy B. coll communis. 



Stock culture .. 

 Race 1 ... 

 Race 2 ... 

 Race 3 ... 

 Race 4 ... 

 Races .">, 8, and 

 Race 6 . . . 

 Race 7 ... 



E. australiana, 

 2nd hour. 1 :600, 



E. austral 'latin,. 

 rect. 1:400. 





'♦ * t\? 



Race 1, the behaviour of which had led to the test, proved to 

 be the most sensitive of all the races. Race 7 which, from its 

 resistance to the 2nd hour oil of E. australiana, promised to be 

 hardy, did not justify the promise either in the second part of 

 the test or in Table ix., where test c. was made with the stock 

 culture and test d. with race 7 upon the same day and at the 

 same time. Race 6 appeared to be the most resistant of all the 

 races, and it was tried with E. australiana (Braidwood) upon 

 the same day and at the same time. The result showed that it 



26 



