3«dim«nt 



Ducard 



Stdimtnt 5*fci« 



Vlatnanoi Wijn 



Cn«d Sadiment 



Methanol 



1 1 1 SOq in Teflon ;ar or ctntrifuge :uoa 

 2) Internal stjnaaraj 

 3)CH30H,CH2C2 11:9) 



'41 rlutfl WfN^ 



«SI Shake it amoient -.emotrature 40 hours .vitri 

 aoivent cnanoa after 16 ana 24 iourj 



CH3OH/CH2C2 

 ixtractl 



(1) NaC! saturated) 



(2) Aodifv v»<Hcl 



3) sxtract 3x «/CH2^ : 2 



Methylene 

 Oionde 

 CH2C2 1 



Aqueous 



Metnanai 



ill Camome 



2) Orv over Ma2S04 



3) Concentrate to 100 -u 

 41 iVeion 



2 scare 



Concentrated 

 extract 



Htxana ifii 

 Weigh Aliduoti 



| 1 ) Oupiact «itn Hexane 



Alumina/ 

 Silica Gd 

 Column 

 Chromatograony 



-lexane/ Methylene 

 Chloride f 21 



Weigh Aliquot) 



Methanol /31 

 Weign Aliouotl 



Saturated 

 rtyoroearoons 



I 



GC 2 

 GC 2 MS 



Aromatic 

 Hydrocaroom 



GC 2 

 GC 2 MS 



foiar MSG 

 Comooundi 



i 



Store 



FIGURE 2.1. Analytical scheme for sediment samples. 



39 



