T.ssue Semoie 

 S50 grams Wet) 



HI Dissect = iesh 



12) Homogenize 



!3) Aod to Teflon Jar 



141 Add Internal Hvoroc»rOon Stanaaros 



(5) 4N KOH laql 



(6) Fiujn Jar witn N^ 

 i7l Saai 



18/ Digest (Saponify* Overmgn: at Room Tsmoereture 



Metnanol ( f 3 ) 

 (Weign) 



Saturated 

 Hydrocarbons 



(1) Aromatic HydrocarDons 



(2) PCS 



Poiar NSO 

 Comoounas 



i 



GC2 



1 



GC2 



GC^/MS 



i 



Store 



Fiaure 2.2. Analytical scheme for tissue samples (after Warner, 1976; Boehm et al., 1982). 



42 



