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Comments 



Auerbach: Did you carry out cytological observations? When I was in 

 Moscow this year, f saw very beautiful preparations made by Prokol- 

 jewa-Belgowskaja, which showed a close correlation between the stages 

 of growth and antibiotic production in Streptomyces and Actinomyces 

 with the appearance of the structure of nuclear bodies. On the basis of 

 these correlations she has been able to advise on the use of culture 

 methods for antibiotic production. 



Nelson: Inspection of stained or unstained mycelia is often used to 

 determine when a pilot plant or production scale vegetative growth 

 tank is ready for use as an inoculum. It has not been used as a method 

 of strain selection since we don't know what changes to look for nor 



