the indophenol technique of Solorzano (34). Salinity measurements were made 

 using an American Optical salinity refractometer. With frequent water changes, 

 most of the water quality parameters changed insignificantly during the course 

 of each experiment. In later experiments where the number of individual 

 treatments became unmanageable, regular monitoring of all variables except 

 temperature was discontinued, and a stringently maintained schedule of water 

 changes observed. 



Feeding larvae were supplied with newly hatched Artemia nauplii at least 

 twice a day in quantities sufficient to permit a portion to remain until the next 

 feeding. Artemia nauplii proved to be a satisfactory diet for striped bass 

 through the early juvenile stage. 



Larval growth was measured in terms of dry weight. Prior to weighing, 

 larvae were lifted on a No. 6 sable brush, dipped in distilled water to remove 

 any adherent salt or debris, blotted on filter paper and placed on a tared 

 weighing pan. Pans were cut out of aluminum foil with a paper punch and 

 ashed 4 hours at 500°C before use. Dry weight determinations were made after 

 the specimen on its tared pan had been dried to a constant weight in a heated 

 vacuum desiccator at 80°C over siHca-gel. On larvae up to metamorphosis, 

 weights were determined using a Cahn "Gram" electrobalance. Weights were 

 read to the nearest microgram. 



Design of Experiments 



Temperature-delayed feeding-survival experiments were performed during 

 the springs of 1976 and 1977. A total of three experiments were performed, 

 each identical in its design. In each, five containers were placed in each of four 

 constant temperature water baths. Each container was stocked with 100 

 prolarvae at the temperature at which they had been held prior to the 

 beginning of the experiment. Stocked containers were assigned to particular 

 temperature treatments by lot. The acclimation period from holding 

 temperature to the experimental temperature treatment was at most one hour. 



In each temperature treatment, the larvae in one container were offered a 

 diet of newly hatched live Artemia nauplii at the beginning of the experiment. 

 Food was withheld from another container throughout the observation period. 

 The time of first feeding for larvae in each of the remaining three containers of 

 starved larvae in each temperature treatment was determined on the basis of 

 observations of the apparent state of health of individuals in each population. 

 Each container was checked for mortality several times each day throughout 

 each experiment, and all dead larvae removed. 



239 



