Table 15-1. Description of the Experimental Diets 



1 

 Experimental Diets Source and Description 



1. Wild plankton Collected in West Passage, Narragansett 



Bay by conical net with 243^/ mesh opening 

 and retained on a 1 16^ mesh sieve. 



2. Brine shrimp San Francisco Bay brine shrimp, Anemia 

 nauplii, live salina, eggs hatched and harvested after 



72 hours in filtered and autoclaved seawater 

 of 20-22°C and 20-30 o/oo salinity. 



3. Starved Unfed 



4. Brine shrimp Nauplii as obtained in diet #2, then 

 nauplii, freeze-dried freeze-dried 24 hours to constant weight. 



5. Tetra Marin' Lot #125244. Tetra Marin Staple Food, 



Tetra Werke Dr. rer. nat. Baensch, Melle, 

 West Germany 



6. Artificial, CM-1 Cortland #1 diet with cod liver oil. 



(See Table 15-2) 



7. Artificial, C-1 Cortland #1 diet with soybean oil. 



(See Table 15-2) 



8. Artificial, CMP-1 Semi-purified diet with cod liver oil. 



(See Table 15-3) 



9. Artificial, CMP-2 Semi-purified diet with cod liver oil and 



an amino acid supplement. (See Table 1 5-3) 



All diets were ground to a coarse powder of 400/i size or less. 



Fish were fed four times daily at a level of five percent body wet weight. A 

 compensation factor was provided to accommodate the flushing action of the 

 system (7). 



Protein Analysis 



All fish were starved for 24 hours prior to sacrificing to reduce the stomach 

 contents. The diet and fish samples were acid hydrolyzed according to the 



217 



