Table 15-2. Composition of the Artificial Diets, 

 CM-1 and C-1 



Components Percent Composition 



CM-1 C-1 



Herring meal 



Soybean oil 



Corn gluten meal, 60% 



Wheat middlings, standard 



Brewers dried yeast 



Dried condensed fish solubles 



Dried Whey 



Meat and Bone Meal 



Soybean oil 



Cod liver oil 



1 

 Mineral mixture 



2 

 Vitamm mixture 



Mixture provided the following compounds in g/kg diet: MgSO^, 2.0; ZnSO^-H^O, 0.3; 

 FeS04-7H20, 0.3; CuSO^, 0.3; KIO3, 0.0091 and MnSO^-H^O, 1.0. 



Mixture provided 10,000 ID Vitamin A as retinyl palmitate; 4,000 lU Vitamin D as 

 Cholecalciferol; 75 lU Vitamin E as dl-a-tocopheryl acetate; and the following amounts 

 (milligrams) of other vitamins per kilogram of diet: menadione dimethylpyriminidol 

 bisulfate, 10.0; thiamine HCL, 4.0; riboflavin, 30.0; calcium pantothenate, 150.0; 

 niacinamide, 300.0; pyridoxine-HCL, 20.0; d-biotin, 6.0; folacin, 15.0; Vitamin B^^' 

 0.002; L-ascorbic acid, 1000; inositol, 500.0; butylated hydroxytoluene (100%), 100.0; 

 and choline chloride (70%), 1330.0. 



methods of Spackman et al (31) and Moore and Stein (27) with modifications 

 by Niederwieser and Pataki (29), Blackburn (10) and Hirs (21). Amino acid 

 analyses were performed on a Technicon Auto Analyzer (NC-2P) with a 25 cm 

 column. An electronic integrator (Columbia Scientific Supergrator 2) was used 

 to compute the absolute amounts of each amino acid. 



Protein content was assayed by microkjeldahl according to Hiller et al (20), 

 and the moisture content was determined using procedures described by 

 Chibnalleffl/(13). 



218 



