Table 15-3. Composition of the Artificial Diets, 

 CMP-1 and CMP-2 



Composition Percent Composition 



CMP-1 CMP-2 



Casein 



Gelatin 



Cod liver oil 



Sucrose 



Dextrin, white Technical 



Cellulose 



Choline chloride (70%) 



L-glutamic-HCI 



L-ascorbic acid 



L-glutamic acid 



NaCI 



2 

 Mineral mixture 



3 

 Vitamin mixture 



DL- Methionine 



L-tryptophan 



Amino Acid mixture 



Solka floe. Brown Company, Berlin, N.H. 



Minerals in g/kg diet: CaHP04H20, 18.04; CaC03, 19.04; KH2PO4, 14.03; NaHC03, 

 8.82; MnS04H20, 0.35; FeS04H20, 0.50; MgSO4,3.02; KIO3, 0.01; CuS04-H20. 

 0.03; ZnC03. 0.15; CsCl2-6H20. 0.002; NaMo04H20, 0.008; and NajSeOg, 0.002. 



The vitamin mixture included 10,000 ID Vitamin A as retinyl palmitate; 4,000 lU 

 Vitamin D as Cholecalciferol; 75 lU Vitamin E as dl-a-tocopheryl acetate; and the 

 following amounts of vitamins in mg/kg diet: thiamin'HCL, 40.0; menadione 

 dimethylpyrimidinol bisulfite (Vitamin K), 2.0; riboflavin, 30.0; D-calcium 

 pantothenate, 150.0; niacin, 300.0; pyridoxine'HCL, 20.0; d-biotin, 0.5; folic acid, 

 15.0; Vitamin B^2' 0-3; Ethoxyquin (100%), 200.0; and myo-inositol, 500.0. 



Amino acids in g/kg diet: L-threonine, 7.0; L-valine, 5.0; L-cystine, 3.0; L-lsoleucine, 

 10.0; L-leucine, 8.0; lysine'HCL, 2.0; and L-arginineHCL, 11.0. 



Lipid Extraction and Analysis 



Samples were collected, weighed, measured, lyophilized, and stored at 

 -20°C under nitrogen. Several small fish or approximately one gram of each 

 diet for each sample were rehydrated with 5 ml distilled water. Samples were 

 extracted twice in a Sorvall Omni-mixer (60 ml capacity), according to the 

 BUgh and Dyer (11) technique as modifed by Kates (23). Lipids were 



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