88 



IIIGII TEMPERATCTRE OUGAXISII OF FERMENTIXU TAX-BARK, 



reacted with the salt, auuulLing its disinfecting action. The constant increase of 

 the seeded over the control flask w.is probably due to the initial activity of the 

 introduced organisms. 



A fermentation experiment was made with tan-bark. Thirty gi-ams of the 

 bark dried at 130° were heated until a thermt)meter inserted in the control flask 

 rose to '200°. then the temperature was slowly reduced to 164° during three- 

 quarters of an iiour and it was kept at this temperature for an hour and a 

 quarter. Altogether the bark was sterilised for two hours at from 164° to 200°. 

 The test flask was treated with 50 c.c. of water containing a suspension of race 

 80 which had been obtained from' the control heated l)ark of the prenous ex- 

 periment. 



Evolution of Carbon Dioxide from Tan-bark. 



On tlie seventh day the nature of tlic curve of the daily yields sliowcd tliat a 

 condition of equilibrium had l>ceu reached and something was needed to accelerate 

 tlie evolution of ga.«. A previous experiment wnth the same bark had shown 

 that alkali acted as such, and accordingly 10 c.c. of alkali were added to test 



