22 : 5/ Thermodynamics of Enzyme Reactions 



415 



TABLE I 



Thermodynamic Denaturation Constants for Various Proteins* 



Substance 



AH* 



AS* 

 (meas. 



' (1) 



Bonds 



broken, 



no.f 



AS* 



(calc. 



Ratio 



(l)/(2) 



* AH* in cal per mole; AS* in cal per mole per degree. 



t Computed from number of broken bonds = A//*/5,000; AS* calc. = (Number of 

 broken bonds) • 12. 



§ AH for denaturation. 



il AS for denaturation. 



After A. E. Stearn, "Kinetics of Biological Reactions with Special Reference to Enzymic 

 Processes," pp. 25-74, in Advances in Enzymology, 9. (New York: Interscience Publishers, 

 Inc., 1949.) 



5. Diffusion Studies 



To properly apply absolute rate theory to enzyme reactions, other than 

 denaturation, it is necessary to know whether the reactions are diffusion 



