provided by the experiments was the extraordinary correlation 

 of mortalities with the cleaning and maintenance cycles. 

 While this helped document the importance of effusions, the 

 same conclusions could be drawn from the bioassays, settlement 

 panels and transect studies. 



7. Biomass Studies : These were difficult and yielded little additional 

 information not gained from other studies. Since the fauna of the 

 Safe Harbor canal walls was impoverished and many of the species 

 living there were rock borers or encrusting organisms the biomass 

 studies required elaborate and not very successful sampling which 

 compromised the analysis. 



8. Diatometers : Glass microscope slides in special racks 

 (diatometers) collected benthic diatoms and protozoans for 

 analyses. They were, however, unsuccessful. I'Jhile collec- 

 tion and analysis of the data were neither time consuming 

 nor costly, variables introduced by filter feeding predators 

 settling on the glass slides reduced the information con- 

 tent of the slides. Two slides could not be satisfactorily 

 compared for differences in benthic diatom or protozoan 

 populations if one was heavily encrusted with filter-feeding 

 serpulids (which competed for space and ate the settling organisms) 

 and the other populated only by diatoms and protozoans. 



9. Plankton Tows : The rapidity with which plankton populations 

 change in nearshore areas limited the information possible 

 from the periodic plankton tows. Daily plankton tows would 

 have required considerable expense in return for conclusions 

 on the impact of the effluent available from other experi- 

 ments. By comparing plankton populations at the various 

 stations with one another, some information was gained which 

 indicated effluent was deleterious to the plankton popula- 

 tions near the discharge. 



10. C^*^ Measurements of Photosynthesis : C^** studies yeilded 

 unusual results. Frequently more carbon was fixed in the 

 dark bottle than in the light. This, in itself, was signi- 

 ficant but a review of the literature suggested the pheno- 

 menon may be a function of the effect of illumination on 

 copper toxicity. The variables that entered into the ex- 

 periment required additional research into the techniques 

 and theory of C^^ measurements which were beyond the scope 

 of the program. 



SUMMARY OF CONCLUSIONS 



In addition to the general conclusions outlined above, the following 

 are pertinent facts obtained during the field work between July, 



