scooped about 100 cc of the upper 1 cm of sediment into Whirl-Pak 

 bags and these were preserved in alcohol. 



Each sediment sample was placed in a 100 ml graduated cylinder and 

 allowed to settle two hours before its volume was recorded. The 

 sample was then wet sifted through a U.S. Standard 63 micron mesh 

 sieve, replaced in the 100 ml graduated cylinder and the new volume 

 recorded. After transfer to a petri dish, an aliquot was spread 

 evenly on a microscope slide until there was only a single layer of 

 sediment. This was examined wet under a AOX compound microscope 

 with transmitted light. A mechanically operated stage permitted 

 the entire slide to be examined systematically. 



The four major species of foraminifera were sorted and a fifth cate- 

 gory, "others" , recorded. Live specimens, characterized by proto- 

 plasm inside the chambers, were recorded separately from dead speci- 

 mens. Aliquots were examined until over 100 live specimens were 

 recorded. The volume of each aliquot was measured in a water-filled 

 1 cc graduated cylinder calibrated to 0.01 cc. 



The number of live specimens in 1 cc of the original sample was 

 calculated from the formula: 



L V 

 L = c s 

 o 



V V 

 c o 



Where L - live foraminifera per cc of original sample, L = number of 



live specimens counted, V = volume examined, V = volume of sifted 



o s 



sample, V = original volume. 



TRANSECTS 



Each month, divers swam two transects; one along the desalination 

 plant sea wall, and the other along the pilings of the City Electric 

 property 393.6 feet (120m) farther into the harbor. Distributions of 

 lobsters (Panulirus argus) , stone crabs (Menippe meroenaria) , sea 

 squirts {Asaidia nigra), bryozoans, serpulids, barnacles, and macro- 

 scopic algae were recorded on plastic slates. Observations included 

 the entire wall of the canal from the inter-tidal zone to the soft 

 sediment about twenty feet from the surface. To equate the data 

 collected on these transects, they were reduced to numbers of organisms 

 per one hundred feet (30m) of sea wall. 



In addition to the transects in Safe Harbor Canal, a series of tran- 

 sects were made both east and west of the turning basin. Ten 100 m 

 transects were made 100 meters apart beginning on the edges of the 

 turning basin as shown in Figure 35. The transects were made using 



32 



