STEP 1 



A series of aquaria containing various dilutions of the effluent in 

 seawater were set up to determine tine 96-hr TLm. Numbers indicate 

 percentage dilution factors. 



20 



20 



STEP 2 



A second series was set up to find the most toxic element of the 

 discharge using the 96-hr TLm dilution determined in Step 1 as a 

 base. Temperature, salinity, and copper were the three factors 

 exami ned . 



tSc 



tsC 



tSC 



tsc 



TSC 



Tsc 



tSc 



tsC 



tSC 



Seawater Effluent at Seawater Seawater Seawater 

 96-hr TLm w/temp. w/salinity w/copper 

 dilution elevated elevated elevated 



Effluent 



w/reduced 



temperature 



96-hr TLm Parameters 

 T = Temperature 

 S = Sa 1 in i ty 

 C = Copper 



Ambient Seawater Parameters 

 t = temperature 

 s = sal in i ty 

 c = copper 



FIG. 7 BIOASSAY EXPERIMENTS 



37 



