The quadrats provided a means of recording chronological changes 

 in the macroinvertebrate fauna. Two of the quadrats were at 

 locations set up in December, 1968. Clarke's (1970) Station 3, 

 directly in the path of the effluent plume, became Station 4. 

 From 1968 to 1971 only a sparce cover of serpulids and barnacles 

 were common in this quadrat. 



Clarke's (1970) Station 2A became Station 3A. There were marked 

 changes in this quadrat from 1968 to 1971 in the number of speci- 

 mens of Asoidia nigra. In December, 1968, there were three per square 

 meter. This value rose to fifteen per square meter by February, 1969 

 and was ten per square meter in May, 1969. In July, 19 70, there were 

 six per square meter and by September, 1970, A. nigra had vanished 

 from the quadrat as well as from the adjacent rock wall. By the end 

 of October, 19 70, A. nigra was gone from all of the entrance canal 

 and inner harbor stations except lA where they persisted until April, 

 1971. In September, 1971, ten specimens of A. nigra were present at 

 Station 3A and in October there were seven specimens. The increase 

 in numbers of A. nigra followed the pronounced drop in ambient dis- 

 solved copper and in copper content in the effluent (figs. 10 and 11). 



The green algae, Cladophoropsis membranaaea, and the red-green algal 

 turf which was abundant in 1968 and during the summer months of 19 70, 

 was missing from 3A from October, 1970 to July, 1971. Cheilostomatid 

 bryozoans {Bugula sp. ) which were common in 1968 and the early summer 

 months of 1970 were absent from March, 1971 until September, 1971. 

 In August, 1970 Branahiomma nigromaaulata, a sabellid worm, became 

 very abundant at Station 3A. In November and December, 19 70 these 

 worms died out in a mass mortality from unknown causes. Populations 

 of B. nigromaaulata did not die in embayments adjacent to Safe Har- 

 bor, nor is the animal known to be seasonal in other Florida areas 

 (Taylor, personal communication). In July, 1971 B. nigromaaulata 

 populations became established again at 3A. In October, 1971, however, 

 relatively few remained. 



From December, 1970 until July, 1971 the fauna at Station 3A consisted 

 primarily of serpulid worms (mostly Hydroides dircmpha) , a few barna- 

 cles (Balanus amphitrite) , and two polychaete worms (Cirriformia 

 filigera and Tharyx marioni) . These organisms maintained good popula- 

 tions at 3A from July, 1970 until October, 1971. 



Station lA was near Clarke's (1970) Station 1 in the inner harbor. 

 A. nigra remained at about the same level of abundance at the inner 

 harbor station from December, 1968 until February, 1971. There were 

 three individuals per square meter in December, 1968; six in January, 

 1969; five in June, 1969; three in July, 1970; five in September, 1970; 

 and seven in December, 1970. By April, however, the number decreased 

 to zero, where it remained until one specimen appeared in October, 1971. 

 Bryozoan colonies remained in good condition at lA through the study 

 period. 



90 



