SECTION XI 

 APPENDIX A 



Procedures of copper analysis (by E.F. Corcoran, Resenstiel 

 School of Marine and Atmospheric Sciences, University of Miami). 



The basic neocuproine techniques used by Alexander and Corcoran 

 (1967) were modified as follows for analyses of water, sediment 

 and organic tissues for copper content: 



1. Glassware Cleanliness : Clean glassware was considered of 

 prime importance and each piece of chemical glassware was 

 washed in a hot solution of Liquinox then rinsed with tap 

 water. To remove remaining detergent, the glassware was 

 rinsed with ethanol and again with tap water. Acid soluble 

 metal ions were removed with acid wash then rinsed with 

 distilled water. A 1 percent solution of disodium salt of 

 EDTA was placed in the flasks and they were autoclaved at 

 248°F at 15 p.s.i. for fifteen minutes. The EDTA solution 

 was removed and the glassware rinsed three times with 

 distilled, deionized water. 



2. Reagents : Sample blanks were kept as low as possible and 

 reagents were selected carefully. 



Distilled water - all reagents were prepared with glass- 

 distilled water further purified by passage through a 

 Barnstead cation exchange resin. 



Neocuproine 0.1% - Ig of neocuproine was dissolved in 1 

 liter of redistilled ethyl alcohol. 



Hydroxylamine hydrochloride 10% - 100 g of hydroxylamine 

 hydrochloride was dissolved in 600 ml of distilled water 

 and filtered through a Whatman GF/C filter pad. Five ml 

 of 0.1% neocuproine were added to the solution and extracted 

 with chloroform. Extractions were continued until the 

 chloroform layer remained colorless. A final extraction 

 was made with carbon tetrachloride and the hydroxylamine 

 hydrochloride solution was diluted to 1 liter. 



Sodium acetate, Crystal reagent grade 27.5% - 453 g of 

 sodium acetate was dissolved in 800 ml distilled water 

 and filtered through a Whatman GF/C filter pad. Two 

 ml of hydroxylamine hydrochloride and 5 ml of 0.1% 

 neocuproine were added. The solution was purified as 



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