196 Hub er: Methocl of preparing' large Nurabcrs of Sections. XXIII, 2. 



bubbles Avhicli collect on the surface of the film. The tray is theu 

 placed so that one of the narrow edges of the plate is toward the 

 manipulator and the film is loosened for a short distauce from this 

 edge and applied to a glass-tube a little longer tlian the width of 

 the film, and aboiit 5 mm to 8 mm in diameter and if the loosened 

 portion of the film has been evenly applied to sides of the glass- 

 tube , the remainder of the film may be readily loosened from the 

 glass-plate and roUed npon the tube as this is caused to rotate over 

 film and plate. Before placiug the dried film into the distilled 

 water , this may be marked as desired witli India ink , or a small 

 Strip of thin })aper , bearing the uecessary information , written in 

 India ink may be placed on the glass-plate and included in the 

 film with the sections. The glass-rods with the photoxyliii films 

 roUed on them may be stored in 80 ^/^ alcohol in large tube vials 

 (18 cm high and 5 cm diameter). If it is desired to unroll a 

 photoxylin film from a glass - tube , this is placed in distilled water 

 when the film may be readily uurolled and stained as desired. 



To Mr. Clarence Snow , the assistant in the laboratory , who 

 has used this method for some time in the preparation of the class 

 material, I am indebted for the working out of many of the details 

 of the method as now used an hcre described. 



References to Literature. 



1) Weigert, Zeitschr. f. wiss. Mikrosk. Bd. II, 1885, p. 490. 



2) Obregia, Neurolog. Zentralbl. Jahrg. IX, 1890, p. 295. 



3) GuLLAND, Journ. Path. and Bact. vol. I, 1893, p. 391. 



4) Strasser, Zeitschr. f. wiss. Mikrosk. Bd. III, 1886, p. 346. 



5) Strasser, Zeitschr. f. wiss. Mikrosk. Bd. IX, 1892, p. 1. 



6) Strasser, Zeitschr. f. wiss. Mikrosk. Bd. XII, 1895, p. 154. 



7) Heidexuaix, Zeitschr. f. wiss. Mikrosk. Bd. XXII, 1905, p. 330. 



8) KüESTER, Zeitschr. f. wiss. Mikrosk. Bd. XVIII, 1901, p. 170. 



9) Weigert, Zeitschr. f. wiss. Mikrosk. Bd. XXI, 1904, p. 1. 



[Eingegangen am 8. Juni 1906.] 



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