328 



The Ohio Journal of Science [Vol. XIX, No. 6, 



Bacterial examinations of the soil in the neighborhood of the 

 growing lupines, conducted by Jasper D. Sayre, have however 

 failed to indicate the presence of the tubercle bacillus in the 

 soil. The ash is extraordinarily poor in micro-organisms. 

 Cultures from numerous collections made in 1917 remained 

 altogether sterile, while in others a single organism developed 

 consistently on some media. Otherwise no micro-organisms 

 whatever were found, although the check samples of garden 

 soil subjected to the same treatment fairly teemed with bacteria, 



f 



K 



■Kit-. :: 



a.2:^^fei:4 . # \ ^J^. 



Photograph by Robert F. Griggs 



WILLOW SEEDLINGS COME UP IN FLOOD BORNE MUD IN KATMAI 



VALLEY. NATURAL SIZE. 



indicating that there was nothing wrong with the methods of 

 collecting and culturing the material. 



The cultural work was interrupted at this stage by con- 

 ditions incident to the war, but through the kindness of Dr. 

 K. F. Kellerman, the Department of Agriculture undertook 

 to investigate our suspicion that the one organism so consistently 

 found was the tubercle bacillus. But when the report came 

 it was negative. Our organism was pronounced not to be 

 Bacillus radicicola. The matter must therefore be left in 



