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flDicro0Copical lEccbniquc, 



Preparing the Ovaries of Scilla patula.— Miss Lily H. Huie 

 finds that the best method for preparing the ovaries of S. patiila^ 

 in order to demonstrate the protein crystalloids, is by first fixing 

 in Mann's Watery Corrosive Fluid. " To a boiling 075 per cent, 

 common salt solution, sublimate is added to saturation (12 grm. 

 for 100 CO.). The solution is then allowed to cool, when crystals 

 of sublimate make their appearance. Preserve the solution with- 

 out decanting. — M. Heidenhain. 



Martin Heidenhain's corrosive sublimate 



solution ... ... ... 100 cc. 



Picric Acid ... ... "... i grm. 



Tannic Acid ... ... ... i grm. 



" The tissues were carefully dehydrated and taken through 

 chloroform into paraffin, and serial sections cut not thicker than 

 2-3 yu- . . The parafiin sections were spread out on warm water 

 (40 — 45'^ C), after Gulland, and fixed to the slide by Mann's 

 albumen method, and then stained in Mann's methylblau-eosine 

 mixture as follows : — 



Requisites. — The staining fluid : — 

 a. — I per cent, methylblau in distilled water ... 35 cc. 

 I per cent, water-soluble eosin in distilled 



water ... ... ... ... 45 cc. 



Distilled water ... ... .. 100 cc, 



b. — I per cent, caustic soda solution in ale. absol. 



The Methylwasserblau (C37H26N3S3O9 Na 3) was obtained 

 from Dr. Griibler, Leipzig. 



Method. 

 I. — Stain for twenty-four hours. 

 2. — Rinse the dark-blue sections in ordinary water. 

 3. — Dehydrate thoroughly with absolute alcohol. 

 4. — Transfer the slide to a vessel containing : Absolute alcohol, 

 30 cc, and i per cent, caustic soda solution in absolute 

 alcohol, 4 drops. Wait till sections are of a rust colour. 

 5. — Remove all traces of caustic soda with absolute alcohol. 



