114 ON SOME PROTEIN CRYSTALLOIDS. 



crystalloids been proved to be in the cytoplasm. Those which 

 form the subject of the present paper are also cytoplasmic, and 

 are found in Scilla patula^ etc. 



Cell-sap Crystalloids. — Under this head it is convenient to 

 group all other known instances of crystalloids occurring in the 

 body of the cell (ii in all, according to Zimmermann), since, with 

 the exceptions just mentioned, whenever their true position has 

 been investigated, they are said to have been found in the Cell-sap. 



Chromatophoric Crystalloids. — Schimper * has already carefully 

 studied the protein crystalloids of Chromatophors, and enumerated 

 the instances of their occurrence, and Zimmermann has extended 

 his researches in this direction. 



Methods. 



Fortner methods. — Zimmermann and Stock fix the material 

 preferably in a saturated solution of corrosive sublimate in abso- 

 lute alcohol ; embed in paraffin and cut sections, which vary in 

 thickness from 5 to lo m- The sections are left for twenty-four 

 hours or more in a o'2 per cent, solution of Saure-fuchsin in dis- 

 tilled water; washed out in running water until sufficiently 

 decolourised ; dehydrated with absolute alcohol, cleared up in 

 xylol, and mounted in xylol-balsam. 



Though both histologists have endeavoured to find other 

 methods for the successful demonstration of these bodies, they 

 have not succeeded in doing so, and come to the conclusion that 

 Saure-fuchsin acts as a specific stain. 



My methods. — The ovaries of Scilla patula were fixed by me 

 in three different ways : — 



I. — In absolute alcohol. 



2. — In Mann's Picro-corrosive Alcohol,t which consists of a 

 saturated solution of corrosive sublimate and picric acid in abso- 

 lute alcohol. 



3. — In Mann's Watery Corrosive Fluid. J 



* Schimper, Untersuchungen iiber die Chlorophyllk'drper, und die thne7i 

 homologen Gehilde, Pringsheim's Jahrbuch, Bd. 1 6, p. i. 



tMann, Trans. Bot. Soc. Edin., vol. xviii., pp. 429, 432, 1890. 

 XJourn. Scot. Micro. Soc, 1894, p. 155. 



