REDEFINE 

 RESEARCH 

 NEE03 ANO 

 GO TO 6A 



toxicity evaluation protocol - laboratory studies 

 i chemical? physical characterization of effluent - 



| CHEMICAL, PHYSICAL CHARACTERIZATION OF RECEIVING SYSTEM - 2 



DETERMINATION OF TESTING PRIORITY -3 



OATA BASE 

 FOR EVALUAT- 

 ING EFFECTS 

 ON AQUATIC 

 LIFE IS 

 DEVELOPEO 



LA80R4TQRY SCREENING TEST - 4A 



I OiHrmmi fh* relonve sensitivity of specific lift history stages of 



fun. frifiBcm and aigae to the chemical substance 



2. Determine the effects of critical environmental parameter* on 

 toxicity fo f<sh, invertebrates, and algae 



3 Ottermine the stability of the toxicoiogicat characteristics of the cherrtcai 

 substance 



4 Sereen the chorrweal subsfoncs for fish flesh fainting. 

 9 Ootermme the potential for bioconcentranon. 



DECISION SOX - 34 



1 Should future studios bt conductod with all three element* of the food chain'* 



2 Should future studios bo conducted lo define the relationship 

 between toxicity and environmental parameters "* 



3. if the chemical substance undergoes chemical, physical or biological 

 degradation producing tone secondary products should future studies 

 bo conductod to define the nature of the chemical substances and the 

 factors affecting it 



4. Are additional fitA flesh tainting tests needed'* 

 9 Is there evidence of pororttioi bioconcentration'* 



NO MORE TESTING 

 REQUIRED UNLESS 

 INOlCATED BY 

 FIELD STUOIES 



DECISION BOX - 7A 



1. Which element of the food chain (i*., fun. invertebrate or algae ) 

 should bo used to evaluate tho effects of sublethal exposure of 

 tho chemical on growth, reproduction and physiology ? 



2. is there adequate information to determine tho eitonf that 

 environmental parameters affect toxicity** 



3. Is fhoro adequate mfarmotion to define the nature of toxicont 

 instability and factors affecting It? 



4.is the concentration causing fish Mesh tainting known ^ 



3. it there a potential for biomagmfication'' 



CHRONIC ANO BIOMAGNIFICATION TESTS - 9A 



1 Determination of tho effects of th* 'oxicant on growth, 

 reproduction and physiology 



2 Determine the effects of environmental paro/nator* m fh* expression 

 of toxicity a* measured by growth, reproduction and physiology 



3 Determination of 'he potential 'or o>omcgnifi cation 



Data base for evoiuatmg effects on aquatic 'e s developed 



Figure 2. Toxicity evaluation protocol — laboratory studies. Reprinted with permission from the American 

 American Society for Testing and Materials as printed in the J. Cairns Jr. and K.L. Dickson. 1978. 

 Field and laboratory protocols for evaluating effects of potentially toxic wastes on aquatic life. 

 J. of Testing and Evaluation 6(2): 35-94. Copyright ASTM. 



168 



