190 



CHARLES OSCAR CHAMBERS 



amount of acid, using 2 cc. instead of 1 cc. I found it best to use 

 only 1 cc. of K2SO4 as a larger amount caused a heavy precipitate 

 of K2SO4 on account of the larger amoimt of NaOH necessary 

 to neutrahze the 2 cc. of H2SO4. On this account, also, it was 

 found necessary to use 10 cc. of water instead of 6 cc. 



TABLE 8 



Experiment G. Aspergillus cultures in Czapek^s solution with 2% dextrose. Each 

 culture contained 5 cc. of the culture solution, to which was added 1 cc. or 2 cc. 

 oj the 1 mgm. ammonia 



TABLE 9 



Experijnent H. Aspergillus cultures in Czapek's solution with 2% dextrose. Each 

 culture contained only 5 cc. of the solution, the ammonia solution being included 

 in the solution when it was made up, so that there was no dilution 



20 cultures were prepared. The others were lost through breakage or other 

 accident. 



