THE DEVELOPMENT OF DUMONTIA 273 



the chromatin is in the form of several spherical bodies which 

 have been formed by a fragmentation of the nucleolus. In 

 Corallina,^ Polysiphonia,^ Delesseria^ and Nitophylliim,^ the 

 chromatin is not contained in the nucleolus, but is present in 

 fine granules distributed along the strands of the linin net of 

 the nucleus. 



The nucleus of the tetrasporangium of Dumontia is surrounded 

 by a jacket of dense cytoplasm. Shortly before division a 

 number of granules appear at the poles of this jacket and in the 

 cytoplasm at the periphery of the cell. These gi'anules are 

 hollow, 1.4m in diameter, and stain sharply with haematoxylin 

 or safranin. They are more numerous where the cytoplasm is 

 more dense and consequently are especially abundant ab the 

 poles of the cytoplasmic jacket enveloping the nucleus (fig. 1, J). 



These granules were tested for fat with Sudan III and cyanin, 

 for starch with chlorozinciodide and potassium iodide, for pro- 

 tein with Millon's reagent. The starch and fat tests were 

 negative and the protein test seemed clearly positive, but as 

 the amount of substance stained was so small and the hght in 

 which it was observed so intense, the red color was very faint. 

 These bodies are evidently a protein substance. After having 

 been treated with Millon's reagent, the granules and nucleolus 

 in the tetrasporangium of Dumontia have the same color. As 

 far as the staining reactions of these granules indicate, since 

 Millon's reagent, haematoxylin, and safranin are nuclein stains, 

 there seems no reason to doubt that they are a nuclein-Uke 

 substance. However, the volume, the position, and time of 

 appearance of these granules is quite convincing evidence that 

 they are not chromatin material. The total volume of all the 



' Davis, B. M. Kerntheilung in Tetrasporen. Mutterzelle bei Corallina 

 officinalis. Ber. d.d. bot. Ges. 16: 266-271. 1898. 



* Yamonouchi, S. The Life History of Polijsiphonia violacea. Bot. Gaz. 

 17: 401-449. 1906. 



» Svedelius, Nils. tJber den Generationswechsel bei Delesseria sanguinea. 

 Svensk Botanisk Tidskrift., Bd. 5, H. 3: 260-324. 1911. 



8 Svodelius, Nils, Ubcr die Tetradenteilung in den vielkernigen Tetrospor- 

 angiumanlagen bei Nitophyllum -punctatum. Ber. d. d. bot. Ges. 32: 452-456. 

 1914. 



